BMRB Entry 15333

Name: Resonance Assignment of the human Pirh2 RING domain; Northeast Structural Genomics Consortium Target HT2B
Published: 2007-07-30

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15333

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance Assignment of the human Pirh2 RING domain; Northeast Structural Genomics Consortium Target HT2B

Deposition date:

2007-06-27

Original release date:

2007-07-30

Authors:

Sheng, Yi; Lemak, Alexander; Laister, Rob; Wu, Bin; Yee, Adelinda; Arrowsmith, Cheryl

Citation:

Citation: Sheng, Yi; Laister, Rob; Lemak, Alexander; Wu, Bin; Tai, Elizabeth; Duran, Shili; Lukin, Jonathan; Sunnerhagen, Maria; Srisailam, Sampath; Karra, Murthy; Benchimol, Sam; Arrowsmith, Cheryl. "Molecular basis of Pirh2-mediated p53 ubiquitylation" Nat. Struct. Mol. Biol. 15, 1334-1342 (2008).
PubMed: 19043414

Assembly members:

Assembly members:
Pirh2_RING_domain, polymer, 52 residues, 6007.025 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pirh2_RING_domain: ENVSQQNCPICLEDIHTSRV VAHVLPCGHLLHRTCYEEML KEGYRCPLCMHS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list

Data type	Count
13C chemical shifts	215
15N chemical shifts	49
1H chemical shifts	345

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Pirh2 RING domain	1
2	ZINC ION, 1	2
3	ZINC ION, 2	2

Entities:

Entity 1, Pirh2 RING domain 52 residues - 6007.025 Da.

1

GLU

ASN

VAL

SER

GLN

ASN

CYS

PRO

ILE

2

CYS

LEU

GLU

ASP

ILE

HIS

THR

SER

ARG

VAL

3

VAL

ALA

HIS

VAL

LEU

PRO

CYS

GLY

HIS

LEU

4

LEU

HIS

ARG

THR

CYS

TYR

GLU

MET

LEU

5

LYS

GLU

GLY

TYR

ARG

CYS

PRO

LEU

CYS

MET

6

HIS

SER

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_2: Pirh2 RING domain, [U-13C; U-15N], 0.7 mM; sodium phosphate 25 mM; potassium chloride 150 mM; ZnCl2 10 uM; DTT, [U-99% 2H], 2 mM; D2O 10%

sample_1: Pirh2 RING domain, [U-13C; U-15N], 0.7 mM; sodium phosphate 25 mM; potassium chloride 150 mM; ZnCl2 10 uM; DTT, [U-99% 2H], 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C_aromatic NOESY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

Bruker Avance 500 MHz
Varian INOVA 600 MHz

PDB	2JRJ
DBJ	BAB31179 BAB31236 BAC37254 BAD92309 BAG52375
GB	AAH23138 AAH47393 AAH57143 AAH83739 AAK96896
REF	NP_001007619 NP_001248474 NP_001258726 NP_001265465 NP_001265467
SP	Q96PM5 Q9CR50
AlphaFold	Q96PM5 Q9CR50