BMRB Entry 15367
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15367
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Citation: Ellison, Michael; Feng, Zhi-Ping; Park, Anthony; Zhang, Xuecheng; Olivera, Baldomero; McIntosh, Michael; Norton, Raymond. "alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR: Structure and
Identification of Key Receptor-Binding Residues" J. Mol. Biol. 377, 1216-1227 (2008).
PubMed: 18295795
Assembly members:
Conotoxin, polymer, 13 residues, 1593.894 Da.
Natural source: Common Name: Conus regius Taxonomy ID: 35631 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus regius
Experimental source: Production method: chemical synthesis Host organism: snail
Entity Sequences (FASTA):
Conotoxin: GCCSEPRCRYRCR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 80 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Conotoxin | 1 |
Entities:
Entity 1, Conotoxin 13 residues - 1593.894 Da.
| 1 | GLY | CYS | CYS | SER | GLU | PRO | ARG | CYS | ARG | TYR | ||||
| 2 | ARG | CYS | ARG |
Samples:
sample_1: toxin 1.0 ± 0.2 mM
sample_conditions_1: pH: 4.22; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization
NMR spectrometers:
- Bruker DRX 600 MHz