BMRB Entry 15391
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15391
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Title: Tetrameric structure of KIA7 peptide PubMed: 17855563
Deposition date: 2007-07-19 Original release date: 2008-03-13
Authors: Lopez de la Osa, Jaime; Gonzalez, Carlos; Laurents, Douglas; Chakrabartty, Avijit; Bateman, David
Citation: Lopez de la Osa, Jaime; Bateman, David; Ho, S.; Gonzalez, Carlos; Chakrabartty, Avijit; Laurents, Douglas. "Getting specificity from simplicity in putative proteins from the prebiotic earth." Proc. Natl. Acad. Sci. U.S.A. 104, 14941-14946 (2007).
Assembly members:
KIA7, polymer, 22 residues, 1869.298 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
KIA7: XAKAAAAAIKAIAAIIKAGG
YX
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 145 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | KIA7 peptide, chain 1 | 1 |
2 | KIA7 peptide, chain 2 | 1 |
3 | KIA7 peptide, chain 3 | 1 |
4 | KIA7 peptide, chain 4 | 1 |
Entities:
Entity 1, KIA7 peptide, chain 1 22 residues - 1869.298 Da.
1 | ACE | ALA | LYS | ALA | ALA | ALA | ALA | ALA | ILE | LYS | ||||
2 | ALA | ILE | ALA | ALA | ILE | ILE | LYS | ALA | GLY | GLY | ||||
3 | TYR | NH2 |
Samples:
KIA7_sample: sodium acetate 10 mM; sodium chloride 200 mM; DSS 0.005 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 278.15 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | KIA7_sample | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | KIA7_sample | isotropic | sample_conditions_1 |
2D DQF-COSY | KIA7_sample | isotropic | sample_conditions_1 |
Software:
AMBER v7.0, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Koll - refinement
DYANA v1.5, Guntert, Braun and Wuthrich - structure solution
NMR spectrometers:
- Bruker DMX 600 MHz
- Bruker Avance 800 MHz