BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15424

Title: NMR Assignment of Cdc42(T35A), and Active Switch I mutant of Cdc42   PubMed: 19636871

Deposition date: 2007-08-08 Original release date: 2007-08-16

Authors: Oswald, Robert

Citation: Adams, Paul; Oswald, Robert. "NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42"  Biomol. NMR Assignments 1, 225-227 (2007).

Assembly members:
Cdc42(T35A), polymer, 178 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):
Cdc42(T35A): MQTIKCVVVGDGAVGKTCLL ISYTTNKFPSEYVPAVFDNY AVTVMIGGEPYTLGLFDTAG QEDYDRLRPLSYPQTDVFLV CFSVVSPSSFENVKEKWVPE ITHHCPKTPFLLVGTQIDLR DDPSTIEKLAKNKQKPITPE TAEKLARDLKAVKYVECSAL TQKGLKNVFDEAILAALE

Data sets:
Data typeCount
13C chemical shifts643
15N chemical shifts172
1H chemical shifts852

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cdc42(T35A) monomer1

Entities:

Entity 1, Cdc42(T35A) monomer 178 residues - Formula weight is not available

1   METGLNTHRILELYSCYSVALVALVALGLY
2   ASPGLYALAVALGLYLYSTHRCYSLEULEU
3   ILESERTYRTHRTHRASNLYSPHEPROSER
4   GLUTYRVALPROALAVALPHEASPASNTYR
5   ALAVALTHRVALMETILEGLYGLYGLUPRO
6   TYRTHRLEUGLYLEUPHEASPTHRALAGLY
7   GLNGLUASPTYRASPARGLEUARGPROLEU
8   SERTYRPROGLNTHRASPVALPHELEUVAL
9   CYSPHESERVALVALSERPROSERSERPHE
10   GLUASNVALLYSGLULYSTRPVALPROGLU
11   ILETHRHISHISCYSPROLYSTHRPROPHE
12   LEULEUVALGLYTHRGLNILEASPLEUARG
13   ASPASPPROSERTHRILEGLULYSLEUALA
14   LYSASNLYSGLNLYSPROILETHRPROGLU
15   THRALAGLULYSLEUALAARGASPLEULYS
16   ALAVALLYSTYRVALGLUCYSSERALALEU
17   THRGLNLYSGLYLEULYSASNVALPHEASP
18   GLUALAILELEUALAALALEUGLU

Samples:

sample_1: Cdc42(T35A), [U-100% 13C; U-100% 15N], 0.3 – 0.5 mM

sample_conditions_1: ionic strength: 0.3 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 4700
PDB
DBJ BAB22563 BAC16312 BAC34669 BAC35825 BAE01909
EMBL CAA90215 CAB57325 CAB57326 CAB57327 CAB57328
GB AAA37410 AAA52494 AAA52592 AAB40051 AAC00027
REF NP_001003254 NP_001008027 NP_001017070 NP_001018130 NP_001034891
SP P60766 P60952 P60953 Q007T2 Q2KJ93
TPG DAA32122
AlphaFold P60952 Q2KJ93 Q007T2 P60953 P60766

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts