BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15447

Title: 8F19F1 module pair from Fibronectin   PubMed: 17921136

Deposition date: 2007-08-29 Original release date: 2007-10-08

Authors: Millard, Chris; Campbell, Iain; Ellis, Ian; Pickford, Andrew; Schor, Anna

Citation: Millard, Chris; Ellis, Ian; Pickford, Andrew; Schor, Ana; Schor, Seth; Campbell, Iain. "The role of the fibronectin IGD motif in stimulating fibroblast migration"  J. Biol. Chem. 282, 35530-35535 (2007).

Assembly members:
8F19F1, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPICZalpha

Entity Sequences (FASTA):
8F19F1: DQCIVDDITYNVNDTFHKKH EEGHMLNCTCFGQGRGRWKC DPVDQCQDSETGTFYQIGDS WEKYVHGVRYQCYCYGRGIG EWHCQPLQ

Data sets:
Data typeCount
15N chemical shifts83
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
18F19F11

Entities:

Entity 1, 8F19F1 88 residues - Formula weight is not available

1   ASPGLNCYSILEVALASPASPILETHRTYR
2   ASNVALASNASPTHRPHEHISLYSLYSHIS
3   GLUGLUGLYHISMETLEUASNCYSTHRCYS
4   PHEGLYGLNGLYARGGLYARGTRPLYSCYS
5   ASPPROVALASPGLNCYSGLNASPSERGLU
6   THRGLYTHRPHETYRGLNILEGLYASPSER
7   TRPGLULYSTYRVALHISGLYVALARGTYR
8   GLNCYSTYRCYSTYRGLYARGGLYILEGLY
9   GLUTRPHISCYSGLNPROLEUGLN

Samples:

sample_1: 8F19F1, [U-15N], 1 mM; D2O, [U-2H], 5%; sodium azide 0.02%; potassium phosphate 20 mM; sodium chloride 150 mM; dioxane 1 mM

sample_2: 8F19F1 1 mM; sodium azide 0.02%; potassium phosphate 20 mM; sodium chloride 150 mM; dioxane 1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian OMEGA 600 MHz
  • Varian OMEGA 500 MHz
  • Varian OMEGA 750 MHz

Related Database Links:

BMRB 15986
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts