BMRB Entry 15468
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15468
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Title: Solution NMR structure of protein NE1242 from N. europaea. Northeast Structural Genomics Target NeT4
Deposition date: 2007-09-12 Original release date: 2007-10-19
Authors: Wu, Yibing; Yee, Adelinda; Zeri, Ana; Guido, Valerie; Sukumaran, Dinesh; Arrowsmith, Cheryl; Szyperski, Thomas
Citation: Wu, Yibing; Yee, Adelinda; Zeri, Ana; Guido, Valerie; Sukumaran, Dinesh; Arrowsmith, Cheryl; Szyperski, Thomas. "Solution NMR structure of protein NE1242 from N. europaea. Northeast Structural Genomics Target NeT4" .
Assembly members:
NE1242, polymer, 73 residues, Formula weight is not available
Natural source: Common Name: Neurospora crassa Taxonomy ID: 5141 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Neurospora crassa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21 DE3
Entity Sequences (FASTA):
NE1242: MTHHTEVFEGGTIDIEDDTS
LTINGKEISYVHDAVKNKWS
SRYLPYTQYDSLLDLARAII
RDTVEFSGVKEGS
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 311 |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 503 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NE1242 | 1 |
Entities:
Entity 1, NE1242 73 residues - Formula weight is not available
| 1 | MET | THR | HIS | HIS | THR | GLU | VAL | PHE | GLU | GLY | ||||
| 2 | GLY | THR | ILE | ASP | ILE | GLU | ASP | ASP | THR | SER | ||||
| 3 | LEU | THR | ILE | ASN | GLY | LYS | GLU | ILE | SER | TYR | ||||
| 4 | VAL | HIS | ASP | ALA | VAL | LYS | ASN | LYS | TRP | SER | ||||
| 5 | SER | ARG | TYR | LEU | PRO | TYR | THR | GLN | TYR | ASP | ||||
| 6 | SER | LEU | LEU | ASP | LEU | ALA | ARG | ALA | ILE | ILE | ||||
| 7 | ARG | ASP | THR | VAL | GLU | PHE | SER | GLY | VAL | LYS | ||||
| 8 | GLU | GLY | SER |
Samples:
sample_1: NE1242 0.5 mM
sample_conditions_1: ionic strength: 0.32 M; pH: .; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 4,3D, GFT HNNCABCA | sample_1 | isotropic | sample_conditions_1 |
| 4,3D, GFT CABCACONNH | sample_1 | isotropic | sample_conditions_1 |
| 4,3D, GFT HCCH COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D, 15N-13C RESOLVEDSIMULTANIOUS NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 4,3D, GFT HABCABCONNH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
AutoStruct, Huang, Tejero, Powers and Montelione - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
XEASY, Bartels et al. - peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts