BMRB Entry 15531

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15531

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Title:
1H 13C and 15N chemical shift for human Granulocyte Macrophage Colony-Stimulating Factor
Deposition date:
2007-10-18
Original release date:
2007-12-10
Authors:
Sauve, Simon; Gingras, Genevieve; Aubin, Yves
Citation:

Citation: Sauve, Simon; Gingras, Genevieve; Aubin, Yves. "NMR assignment of human granulocyte-macrophage colony-stimulating factor"  Biomol. NMR Assignments 2, 5-7 (2008).
PubMed: 19636911

Assembly members:

Assembly members:
rhGM-CSF, polymer, 127 residues, 14477.5 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b10

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rhGM-CSF: APARSPSPSTQPWEHVNAIQ EARRLLNLSRDTAAEMNETV EVISEMFDLQEPTCLQTRLE LYKQGLRGSLTKLKGPLTMM ASHYKQHCPPTPETSCATQI ITFESFKENLKDFLLVIPFD CWEPVQE

Data sets:
Data typeCount
13C chemical shifts474
15N chemical shifts122
1H chemical shifts554

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1molecule 11

Entities:

Entity 1, molecule 1 127 residues - 14477.5 Da.

1   ALAPROALAARGSERPROSERPROSERTHR
2   GLNPROTRPGLUHISVALASNALAILEGLN
3   GLUALAARGARGLEULEUASNLEUSERARG
4   ASPTHRALAALAGLUMETASNGLUTHRVAL
5   GLUVALILESERGLUMETPHEASPLEUGLN
6   GLUPROTHRCYSLEUGLNTHRARGLEUGLU
7   LEUTYRLYSGLNGLYLEUARGGLYSERLEU
8   THRLYSLEULYSGLYPROLEUTHRMETMET
9   ALASERHISTYRLYSGLNHISCYSPROPRO
10   THRPROGLUTHRSERCYSALATHRGLNILE
11   ILETHRPHEGLUSERPHELYSGLUASNLEU
12   LYSASPPHELEULEUVALILEPROPHEASP
13   CYSTRPGLUPROVALGLNGLU

Samples:

sample_1: rhGM-CSF, [U-99% 13C; U-99% 15N], 400 uM; sodium phosphate 2 mM; DSS 1 mM; D2O 5%

sample_conditions_1: ionic strength: 2 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D C(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D C(CO)NH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v7.0.16, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

Smartnotebook v5.0.6, Brian Sykes - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Varian INOVA 600 MHz
  • Bruker Avance III 600 MHz

Related Database Links:

PDB
DBJ BAI23240 BAJ21159
EMBL CAA26822
GB AAA52121 AAA52122 AAA52578 AAA98768 AAC08707
REF NP_000749 XP_002815902 XP_003829363 XP_004042512 XP_527005
SP P04141
AlphaFold P04141

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks