BMRB Entry 15539

Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15539

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Title:
Backbone 1H, 13C, and 15N NMR assignments for the Cyanothece 51142 protein A1KYE3 - a protein in the DUF683 family associated with nitrogen fixation.
Deposition date:
2007-10-28
Original release date:
2008-01-17
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Sofia, Heidi. "Backbone 1H, 13C, and 15N NMR assignments for the Cyanothece 51142 protein cce_0567: a protein associated with nitrogen fixation in the DUF683 family"  Biomol. NMR Assignments 2, 25-28 (2008).
PubMed: 19636916

Assembly members:

Assembly members:
DUF683_containing_polypeptide, polymer, 81 residues, 37000 Da.

Natural source:

Natural source:   Common Name: Cyanothece 51142   Taxonomy ID: 43989   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Cyanothece Cyanothece sp. ATCC 51142

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DUF683_containing_polypeptide: GSHMTVATDNNPTPEAVADL KKKVRKLNSKAGQMKMDLHD LAEGLPTDYENLVETAEKTY EIFRELDQLKKKLNIWEETL K

Data sets:
Data typeCount
13C chemical shifts248
15N chemical shifts75
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Polypeptide 11
2Polypeptide 21
3Polypeptide 31
4Polypeptide 41

Entities:

Entity 1, Polypeptide 1 81 residues - 37000 Da.

Residues 1-3 remain after cleavage with thrombin. Fourth residue is M1.

1   GLYSERHISMETTHRVALALATHRASPASN
2   ASNPROTHRPROGLUALAVALALAASPLEU
3   LYSLYSLYSVALARGLYSLEUASNSERLYS
4   ALAGLYGLNMETLYSMETASPLEUHISASP
5   LEUALAGLUGLYLEUPROTHRASPTYRGLU
6   ASNLEUVALGLUTHRALAGLULYSTHRTYR
7   GLUILEPHEARGGLULEUASPGLNLEULYS
8   LYSLYSLEUASNILETRPGLUGLUTHRLEU
9   LYS

Related Database Links:

PDB 3CSX
GB AAW56996 ACB49918
REF WP_009546647

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks