BMRB Entry 15653

Assembly:

Entities:

Entity 1, Vinculin Tail 192 residues - Formula weight is not available

Residues 1-4 are non-native. Residues 5-192 represent residues 879-1066 of chicken vinculin, commonly refered to as vinculin tail (Vt). Chicken Vt shares 98.9% identity with human Vt.

Samples:

sample_1: Vinculin Tail, [U-13C; U-15N; U-2H], 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_2: Vinculin Tail, [U-13C; U-15N], 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_3: Vinculin Tail, [U-15N]-Leu, 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_4: Vinculin Tail, [U-15N]-Met, 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_5: Vinculin Tail, [U-15N; NA-Lys], 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 310 K

Experiments:

Software:

NMRView v5.2.2.01, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe/NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

• Varian INOVA 500 MHz
• Varian INOVA 600 MHz
• Varian INOVA 700 MHz
• Varian INOVA 800 MHz