BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15719

Title: Assignment of the central region (131-28) of human h1 calponin   PubMed: 21463585

Deposition date: 2008-04-07 Original release date: 2008-05-05

Authors: Pfuhl, Mark; El Mezgueldi, Mohammed

Citation: Pfuhl, Mark; Al-Sarayreh, Sameeh; El-Mezgueldi, Mohammed. "The calponin regulatory region is intrinsically unstructured: novel insight into actin-calponin and calmodulin-calponin interfaces using NMR spectroscopy."  Biophys. J. 100, 1718-1728 (2011).

Assembly members:
calponin, polymer, 98 residues, 10807.3 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):
calponin: AKTKGNKVNVGVKYAEKQER RFEPEKLREGRNIIGLQMGI NKFASQQGMTAYGTRRHLYD PKLGTDQPLDQATISLQMGT NKGASQAGMTAPGTKRQI

Data sets:
Data typeCount
13C chemical shifts307
15N chemical shifts83
1H chemical shifts425

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calponin h1 131-2281

Entities:

Entity 1, calponin h1 131-228 98 residues - 10807.3 Da.

1   ALALYSTHRLYSGLYASNLYSVALASNVAL
2   GLYVALLYSTYRALAGLULYSGLNGLUARG
3   ARGPHEGLUPROGLULYSLEUARGGLUGLY
4   ARGASNILEILEGLYLEUGLNMETGLYILE
5   ASNLYSPHEALASERGLNGLNGLYMETTHR
6   ALATYRGLYTHRARGARGHISLEUTYRASP
7   PROLYSLEUGLYTHRASPGLNPROLEUASP
8   GLNALATHRILESERLEUGLNMETGLYTHR
9   ASNLYSGLYALASERGLNALAGLYMETTHR
10   ALAPROGLYTHRLYSARGGLNILE

Samples:

sample_1: calponin, [U-100% 15N], 1.5 ± 0.1 mM; D2O 5 ± 0.1 %; H2O 95 ± 0.1 %; DTT 2 ± 0.05 mM; potassium phosphate 20 ± 1 mM; sodium chloride 20 ± 1 mM; sodium azide 0.02 ± 0.0005 %

sample_2: calponin, [U-100% 13C; U-100% 15N], 1.0 ± 0.1 mM; D2O 5 ± 0.1 %; H2O 95 ± 0.1 %; DTT 2 ± 0.05 mM; potassium phosphate 20 ± 1 mM; sodium chloride 20 ± 1 mM; sodium azide 0.02 ± 0.0005 %

sample_conditions_1: ionic strength: 0.4 M; pH: 7.0; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D (H)C(CO)NHsample_2isotropicsample_conditions_1
3D HBHA(CBCA)NHsample_2isotropicsample_conditions_1
3D HBHA(CBCACO)NHsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - processing

CCPN_analysis v1.1, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

GB AAF18235 EDL25252 EDL78231

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts