BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15739

Title: Hydramacin-1: Structure and antibacterial activity of a peptide from the basal metazoan Hydra   PubMed: 19019828

Deposition date: 2008-04-22 Original release date: 2008-11-25

Authors: Jung, Sascha; Dingley, Andrew; Stanisak, Mareike; Gelhaus, Christoph; Bosch, Thomas; Podschun, Rainer; Leippe, Matthias; Groetzinger, Joachim

Citation: Jung, Sascha; Dingley, Andrew; Augustin, Rene; Anton-Erxleben, Frederike; Stanisak, Mareike; Gelhaus, Christoph; Gutsmann, Thomas; Hammer, Malte; Podschun, Rainer; Bonvin, Alexandre; Leippe, Matthias; Bosch, Thomas; Groetzinger, Joachim. "Hydramacin-1: Structure and antibacterial activity of a protein from the basal metazoan hydra"  J. Biol. Chem. 284, 1896-1905 (2009).

Assembly members:
hydramacin-1, polymer, 60 residues, Formula weight is not available

Natural source:   Common Name: Hydra   Taxonomy ID: 6083   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hydra not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):
hydramacin-1: QIVDCWETWSRCTKWSQGGT GTLWKSCNDRCKELGRKRGQ CEEKPSRCPLSKKAWTCICY

Data typeCount
13C chemical shifts172
15N chemical shifts62
1H chemical shifts340

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hydramacin-11

Entities:

Entity 1, hydramacin-1 60 residues - Formula weight is not available

1   GLNILEVALASPCYSTRPGLUTHRTRPSER
2   ARGCYSTHRLYSTRPSERGLNGLYGLYTHR
3   GLYTHRLEUTRPLYSSERCYSASNASPARG
4   CYSLYSGLULEUGLYARGLYSARGGLYGLN
5   CYSGLUGLULYSPROSERARGCYSPROLEU
6   SERLYSLYSALATRPTHRCYSILECYSTYR

Samples:

sample_1: hydramacin-1, [U-100% 13C; U-100% 15N], 1.0 mM; D2O 7%; H2O 93%

sample_conditions_1: ionic strength: 0.050 M; pH: 5.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB ABE26989
SP B3RFR8
AlphaFold B3RFR8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts