BMRB Entry 15751
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15751
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Citation: Vasudevan, Sheeja; Yuan, Jun; Osapay, George; Tran, Patti; Tai, Kenneth; Liang, Warren; Kumar, Vasanth; Selsted, Michael; Cocco, Melanie. "Synthesis, structure and activities of an oral mucosal alpha-defensin from rhesus macaque" J. Biol. Chem. 283, 35869-35877 (2008).
PubMed: 18930922
Assembly members:
ROAD-1, polymer, 32 residues, 3754.392 Da.
Natural source: Common Name: rhesus monkey Taxonomy ID: 9544 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: rhesus macaque
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
ROAD-1: RRTCHCRSRCLRRESNSGSC
NINGRIFSLCCR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 188 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ROAD-1 | 1 |
Entities:
Entity 1, ROAD-1 32 residues - 3754.392 Da.
| 1 | ARG | ARG | THR | CYS | HIS | CYS | ARG | SER | ARG | CYS | ||||
| 2 | LEU | ARG | ARG | GLU | SER | ASN | SER | GLY | SER | CYS | ||||
| 3 | ASN | ILE | ASN | GLY | ARG | ILE | PHE | SER | LEU | CYS | ||||
| 4 | CYS | ARG |
Samples:
sample_1: ROAD-1 0.3 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 3.8; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRDraw v2.2, CCPN - processing
Analysis v1.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment
NMR spectrometers:
- Varian INOVA 800 MHz