BMRB Entry 15756
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15756
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Title: 1H, 13C and 15N backbone chemical shift assignments of the 2FNI-3FNI module pair from human fibronectin PubMed: 19366708
Deposition date: 2008-05-06 Original release date: 2009-04-15
Authors: Vakonakis, Ioannis; Campbell, Iain
Citation: Vakonakis, Ioannis; Staunton, David; Ellis, Ian; Sarkies, Peter; Flanagan, Aleksandra; Schor, Ana; Schor, Seth; Campbell, Iain. "Motogenic sites in human fibronectin are masked by long range interactions" J. Biol. Chem. 284, 15668-15675 (2009).
Assembly members:
2F13F1, polymer, 89 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPIC9K
Entity Sequences (FASTA):
2F13F1: EETCFDKYTGNTYRVGDTYE
RPKDSMIWDCTCIGAGRGRI
SCTIANRCHEGGQSYKIGDT
WRRPHETGGYMLECVCLGNG
KGEWTCKPI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 160 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 88 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | protein | 1 |
Entities:
Entity 1, protein 89 residues - Formula weight is not available
| 1 | GLU | GLU | THR | CYS | PHE | ASP | LYS | TYR | THR | GLY | ||||
| 2 | ASN | THR | TYR | ARG | VAL | GLY | ASP | THR | TYR | GLU | ||||
| 3 | ARG | PRO | LYS | ASP | SER | MET | ILE | TRP | ASP | CYS | ||||
| 4 | THR | CYS | ILE | GLY | ALA | GLY | ARG | GLY | ARG | ILE | ||||
| 5 | SER | CYS | THR | ILE | ALA | ASN | ARG | CYS | HIS | GLU | ||||
| 6 | GLY | GLY | GLN | SER | TYR | LYS | ILE | GLY | ASP | THR | ||||
| 7 | TRP | ARG | ARG | PRO | HIS | GLU | THR | GLY | GLY | TYR | ||||
| 8 | MET | LEU | GLU | CYS | VAL | CYS | LEU | GLY | ASN | GLY | ||||
| 9 | LYS | GLY | GLU | TRP | THR | CYS | LYS | PRO | ILE |
Samples:
sample_1: 2F13F1, [U-99% 13C; U-99% 15N], 0.3 mM; D2O 5%; DSS 0.1 mM; sodium azide 0.01%; sodium phosphate 20 mM; sodium chloride 20 mM
sample_conditions_1: ionic strength: 115 mM; pH: 6; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
PIPP v4.3.7, Garrett - data analysis
Omega Spectrometer Operating Software v6.0.3b2, Bruker Instruments - collection
NMR spectrometers:
- N/A N/A 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts