BMRB Entry 15758
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15758
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Title: 1H, 13C and 15N backbone chemical shift assignments of the 4FNI-5FNI module pair from human fibronectin PubMed: 19366708
Deposition date: 2008-05-06 Original release date: 2009-04-15
Authors: Vakonakis, Ioannis; Campbell, Iain
Citation: Vakonakis, Ioannis; Staunton, David; Ellis, Ian; Sarkies, Peter; Flanagan, Aleksandra; Schor, Ana; Schor, Seth; Campbell, Iain. "Motogenic sites in human fibronectin are masked by long range interactions" J. Biol. Chem. 284, 15668-15675 (2009).
Assembly members:
4F15F1, polymer, 93 residues, 10519.6 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPIC9K
Entity Sequences (FASTA):
4F15F1: AEKCFDHAAGTSYVVGETWE
KPYQGWMMVDCTCLGEGSGR
ITCTSRNRCNDQDTRTSYRI
GDTWSKKDNRGNLLQCICTG
NGRGEWKCERHTS
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 175 |
15N chemical shifts | 96 |
1H chemical shifts | 98 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | protein | 1 |
Entities:
Entity 1, protein 93 residues - 10519.6 Da.
1 | ALA | GLU | LYS | CYS | PHE | ASP | HIS | ALA | ALA | GLY | ||||
2 | THR | SER | TYR | VAL | VAL | GLY | GLU | THR | TRP | GLU | ||||
3 | LYS | PRO | TYR | GLN | GLY | TRP | MET | MET | VAL | ASP | ||||
4 | CYS | THR | CYS | LEU | GLY | GLU | GLY | SER | GLY | ARG | ||||
5 | ILE | THR | CYS | THR | SER | ARG | ASN | ARG | CYS | ASN | ||||
6 | ASP | GLN | ASP | THR | ARG | THR | SER | TYR | ARG | ILE | ||||
7 | GLY | ASP | THR | TRP | SER | LYS | LYS | ASP | ASN | ARG | ||||
8 | GLY | ASN | LEU | LEU | GLN | CYS | ILE | CYS | THR | GLY | ||||
9 | ASN | GLY | ARG | GLY | GLU | TRP | LYS | CYS | GLU | ARG | ||||
10 | HIS | THR | SER |
Samples:
sample_1: 4F15F1, [U-98% 13C; U-98% 15N], 0.2 mM; D2O 5%; sodium azide 0.01%; sodium chloride 20 mM; sodium phosphate 20 mM; DSS 0.1 mM
sample_conditions_1: ionic strength: 115 mM; pH: 6; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
PIPP v4.3.7, Garrett - data analysis
TOPSPIN v1.3, Bruker Biospin - collection
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts