BMRB Entry 15831
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15831
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Title: chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y2-receptor PubMed: 19125388
Deposition date: 2008-06-27 Original release date: 2009-02-13
Authors: Zerbe, Oliver; Walser, Reto; Zou, Chao; Kumaran, Sowmini
Citation: Zou, Chao; Kumaran, Sowmini; Walser, Reto; Zerbe, Oliver. "Properties of the N-terminal domains from Y receptors probed by NMR spectroscopy." J. Pept. Sci. 15, 184-191 (2009).
Assembly members:
N-Y2, polymer, 50 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pUBK19
Entity Sequences (FASTA):
N-Y2: MGPIGAEADENQTVEEMKVE
QYGPQTTPRGELVPDPEPEL
IDSTKLIEVQ
- assigned_chemical_shifts
- heteronucl_NOEs
Data type | Count |
15N chemical shifts | 43 |
1H chemical shifts | 43 |
heteronuclear NOE values | 43 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | N-Y2 | 1 |
Entities:
Entity 1, N-Y2 50 residues - Formula weight is not available
1 | MET | GLY | PRO | ILE | GLY | ALA | GLU | ALA | ASP | GLU | |
2 | ASN | GLN | THR | VAL | GLU | GLU | MET | LYS | VAL | GLU | |
3 | GLN | TYR | GLY | PRO | GLN | THR | THR | PRO | ARG | GLY | |
4 | GLU | LEU | VAL | PRO | ASP | PRO | GLU | PRO | GLU | LEU | |
5 | ILE | ASP | SER | THR | LYS | LEU | ILE | GLU | VAL | GLN |
Samples:
sample_1: N-Y2, [U-15N], 0.2 mM; MES 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_2: N-Y2, [U-15N], 0.4 mM; MES 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N NOE | sample_2 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
DBJ | BAG74149 |
GB | AAA93170 AAB04120 AAB07760 AAC50281 AAC51115 |
REF | NP_000901 NP_001012655 NP_001028004 XP_002832879 XP_003257918 |
SP | P49146 Q5IS62 Q9GK74 |
AlphaFold | Q5IS62 Q9GK74 P49146 |
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