BMRB Entry 15881

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15881

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Title:
NMR assignment of Prespore Specific Antigen - a cell surface adhesion glycoprotein from Dictyostelium discoideum
Deposition date:
2008-07-20
Original release date:
2008-11-17
Authors:
Swarbrick, James; Mabbutt, Bridget
Citation:

Citation: Swarbrick, James; Cubeddu, Liza; Ball, Graham; Curmi, Paul; Gooley, Andrew; Williams, Keith; Mabbutt, Bridget. "NMR assignment of prespore specific antigen--a cell surface adhesion glycoprotein from Dictyostelium discoideum"  Biomol. NMR Assignments 3, 1-3 (2009).
PubMed: 19636933

Assembly members:

Assembly members:
PSA, polymer, 122 residues, Formula weight is not available
NAG, non-polymer, 221.208 Da.

Natural source:

Natural source:   Common Name: Dictyostelium discoideum   Taxonomy ID: 44689   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Dictyostelium discoideum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Dictyostelium discoideum   Vector: -

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PSA: YDYFTTTLANQNPVCASVDV IQNVCTEVCGRFVRYIPDAT NTNQFTFAEYTTNQCTVQVT PAVTNTFTCADQTSSHALGS DWSGVCKITATPAPTVTPTV TPTVTPTVTPTPTNTPNPTP SK

Data sets:
Data typeCount
13C chemical shifts470
15N chemical shifts138
1H chemical shifts794

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PSA1
2NAG, 12
3NAG, 22
4NAG, 32
5NAG, 42
6NAG, 52
7NAG, 62
8NAG, 72
9NAG, 82
10NAG, 92
11NAG, 102
12NAG, 112

Entities:

Entity 1, PSA 122 residues - Formula weight is not available

1   TYRASPTYRPHETHRTHRTHRLEUALAASN
2   GLNASNPROVALCYSALASERVALASPVAL
3   ILEGLNASNVALCYSTHRGLUVALCYSGLY
4   ARGPHEVALARGTYRILEPROASPALATHR
5   ASNTHRASNGLNPHETHRPHEALAGLUTYR
6   THRTHRASNGLNCYSTHRVALGLNVALTHR
7   PROALAVALTHRASNTHRPHETHRCYSALA
8   ASPGLNTHRSERSERHISALALEUGLYSER
9   ASPTRPSERGLYVALCYSLYSILETHRALA
10   THRPROALAPROTHRVALTHRPROTHRVAL
11   THRPROTHRVALTHRPROTHRVALTHRPRO
12   THRPROTHRASNTHRPROASNPROTHRPRO
13   SERLYS

Entity 2, NAG, 1 - C8 H15 N O6 - 221.208 Da.

1   NAG

Samples:

sample_1: PSA, [U-99% 13C; U-99% 15N], 1.0 mM; Acetate 30 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.030 M; pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H TOCSY RELAYED CT [13c,1h] HMQCsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

EMBL CAA34102
GB AAA52100 EAL73158
PRF 1615147A
REF XP_647406
SP P12729
AlphaFold P12729

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks