BMRB Entry 15883
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15883
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Title: Backbone and 13C and 1H assignments for acid unfolded state and urea unfolded state of CTL9 PubMed: 18707127
Deposition date: 2008-07-24 Original release date: 2008-09-19
Authors: Shan, Bing; Bhattacharya, Shibani; Eliezer, David; Raleigh, Daniel
Citation: Shan, Bing; Bhattacharya, Shibani; Eliezer, David; Raleigh, Daniel. "The Low pH Unfolded State of the C-terminal Domain of the Ribosomal Protein L9 Contains Significant Secondary Structure in the Absence of Denaturant but is No More Compact than the Low pH Urea Unfolded State" Biochemistry 47, 9565-9573 (2008).
Assembly members:
CTL9, polymer, 92 residues, Formula weight is not available
Natural source: Common Name: Bacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus stearothermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a
Entity Sequences (FASTA):
CTL9: AAEELANAKKLKEQLEKLTV
TIPAKAGEGGRLFGSITSKQ
IAESLQAQHGLKLDKRKIEL
ADAIRALGYTNVPVKLHPEV
TATLKVHVTEQK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 267 |
15N chemical shifts | 88 |
1H chemical shifts | 290 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | CTL9 | 1 |
Entities:
Entity 1, CTL9 92 residues - Formula weight is not available
1 | ALA | ALA | GLU | GLU | LEU | ALA | ASN | ALA | LYS | LYS | ||||
2 | LEU | LYS | GLU | GLN | LEU | GLU | LYS | LEU | THR | VAL | ||||
3 | THR | ILE | PRO | ALA | LYS | ALA | GLY | GLU | GLY | GLY | ||||
4 | ARG | LEU | PHE | GLY | SER | ILE | THR | SER | LYS | GLN | ||||
5 | ILE | ALA | GLU | SER | LEU | GLN | ALA | GLN | HIS | GLY | ||||
6 | LEU | LYS | LEU | ASP | LYS | ARG | LYS | ILE | GLU | LEU | ||||
7 | ALA | ASP | ALA | ILE | ARG | ALA | LEU | GLY | TYR | THR | ||||
8 | ASN | VAL | PRO | VAL | LYS | LEU | HIS | PRO | GLU | VAL | ||||
9 | THR | ALA | THR | LEU | LYS | VAL | HIS | VAL | THR | GLU | ||||
10 | GLN | LYS |
Samples:
sample_1: CTL9, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: pH: 2.0; pressure: 1 atm; temperature: 298.2 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Zhengrong and Bax - processing
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 700 MHz
Related Database Links:
BMRB | 15884 17460 |
PDB | |
EMBL | CAA43972 |
GB | AAA22701 AKM20686 AKU26730 KFL16938 KFX34457 |
PRF | 0701226A 1714237A |
REF | WP_033008865 WP_050368025 |
SP | P02417 |
AlphaFold | P02417 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts