BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15909

Title: 1J coupling constants related to the Ca carbons in DFPase from Loligo vulgaris   PubMed: 18853398

Deposition date: 2008-08-04 Original release date: 2008-11-19

Authors: Schmidt, Jurgen; Lohr, Frank

Citation: Schmidt, Jurgen; Howard, Mark; Maestre-Martinez, Mitcheell; Perez, Carlos; Lohr, Frank. "Variation in protein C(alpha)-related one-bond J couplings"  Magn. Reson. Chem. 47, 16-30 (2009).

Assembly members:
DFPase, polymer, 316 residues, 35081.3 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Loligo vulgaris   Taxonomy ID: 6622   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Loligo vulgaris

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKKHisND

Entity Sequences (FASTA):
DFPase: GSMEIPVIEPLFTKVTEDIP GAEGPVFDKNGDFYIVAPEV EVNGKPAGEILRIDLKTGKK TVICKPEVNGYGGIPAGCQC DRDANQLFVADMRLGLLVVQ TDGTFEEIAKKDSEGRRMQG CNDCAFDYEGNLWITAPAGE VAPADYTRSMQEKFGSIYCF TTDGQMIQVDTAFQFPNGIA VRHMNDGRPYQLIVAETPTK KLWSYDIKGPAKIENKKVWG HIPGTHEGGADGMDFDEDNN LLVANWGSSHIEVFGPDGGQ PKMRIRCPFEKPSNLHFKPQ TKTIFVTEHENNAVWKFEWQ RNGKKQYCETLKFGIF

Data sets:
Data typeCount
coupling constants780

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2CALCIUM ION, 12
3CALCIUM ION, 22

Entities:

Entity 1, protein 316 residues - 35081.3 Da.

Leading residues Gly(-2) and Ser(-1) are cloning artefacts and do not give NMR signals.

1   GLYSERMETGLUILEPROVALILEGLUPRO
2   LEUPHETHRLYSVALTHRGLUASPILEPRO
3   GLYALAGLUGLYPROVALPHEASPLYSASN
4   GLYASPPHETYRILEVALALAPROGLUVAL
5   GLUVALASNGLYLYSPROALAGLYGLUILE
6   LEUARGILEASPLEULYSTHRGLYLYSLYS
7   THRVALILECYSLYSPROGLUVALASNGLY
8   TYRGLYGLYILEPROALAGLYCYSGLNCYS
9   ASPARGASPALAASNGLNLEUPHEVALALA
10   ASPMETARGLEUGLYLEULEUVALVALGLN
11   THRASPGLYTHRPHEGLUGLUILEALALYS
12   LYSASPSERGLUGLYARGARGMETGLNGLY
13   CYSASNASPCYSALAPHEASPTYRGLUGLY
14   ASNLEUTRPILETHRALAPROALAGLYGLU
15   VALALAPROALAASPTYRTHRARGSERMET
16   GLNGLULYSPHEGLYSERILETYRCYSPHE
17   THRTHRASPGLYGLNMETILEGLNVALASP
18   THRALAPHEGLNPHEPROASNGLYILEALA
19   VALARGHISMETASNASPGLYARGPROTYR
20   GLNLEUILEVALALAGLUTHRPROTHRLYS
21   LYSLEUTRPSERTYRASPILELYSGLYPRO
22   ALALYSILEGLUASNLYSLYSVALTRPGLY
23   HISILEPROGLYTHRHISGLUGLYGLYALA
24   ASPGLYMETASPPHEASPGLUASPASNASN
25   LEULEUVALALAASNTRPGLYSERSERHIS
26   ILEGLUVALPHEGLYPROASPGLYGLYGLN
27   PROLYSMETARGILEARGCYSPROPHEGLU
28   LYSPROSERASNLEUHISPHELYSPROGLN
29   THRLYSTHRILEPHEVALTHRGLUHISGLU
30   ASNASNALAVALTRPLYSPHEGLUTRPGLN
31   ARGASNGLYLYSLYSGLNTYRCYSGLUTHR
32   LEULYSPHEGLYILEPHE

Entity 2, CALCIUM ION, 1 - Ca - 40.078 Da.

1   CA

Samples:

doubly_labeled: DFPase, [U-95% 13C; U-95% 15N], 1.6 mM; calcium chloride 5 mM; sodium azide 0.03%; Pefabloc protease inhibitor 50 ug/ml; D2O, [U-2H], 5%; H2O 95%

triply_labeled: DFPase, [U-95% 13C; U-95% 15N; 90% 2H], 0.75 mM; calcium chloride 5 mM; sodium azide 0.03%; Pefabloc protease inhibitor 50 ug/ml; D2O, [U-2H], 5%; H2O 95%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
3D Ca-coupled [15N,1H]-TROSY-HNCOtriply_labeledisotropicsample_conditions_1
3D Cb-coupled [15N,1H]-TROSY-HN(CO)CAtriply_labeledisotropicsample_conditions_1
2D IPAP-type HN(CO-a/b-NCa-J)-TROSYtriply_labeledisotropicsample_conditions_1
3D quantitative J-correlated [15N,1H]-TROSY-HNCtriply_labeledisotropicsample_conditions_1
3D Ca-coupled HA(CACO)NHdoubly_labeledisotropicsample_conditions_1
3D Ha-coupled [15N,1H]-TROSY-HNCAdoubly_labeledisotropicsample_conditions_1
3D Ha-coupled [15N,1H]-TROSY-iHNCAdoubly_labeledisotropicsample_conditions_1
3D Ha-coupled [15N,1H]-TROSY-HN(CO)CAdoubly_labeledisotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection, processing

jeval, JM Schmidt - coupling constant extraction, data analysis, multiplet simulation

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker Avance 700 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 16584
PDB
SP Q7SIG4
AlphaFold Q7SIG4