BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15942

Title: 1H, 13C and 15N assignments for rrm2 domain of PABP1

Deposition date: 2008-09-05 Original release date: 2012-07-19

Authors: Barsukov, Igor; Parnham, Stuart

Citation: Barsukov, Igor; Parnham, Stuart; Norman, Jim. "Solution structure of RRM2 domain of PABP1"  .

Assembly members:
PABP1, polymer, 95 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: petM-11

Entity Sequences (FASTA):
PABP1: GADPSLRKSGVGNIFIKNLD KSIDNKALYDTFSAFGNILS CKVVCDENGSKGYGFVHFET QEAAERAIEKMNGMLLNDRK VFVGRFKSRKEREAE

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts100
1H chemical shifts660

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM21

Entities:

Entity 1, RRM2 95 residues - Formula weight is not available

residues 1 and 2 are from TEV cleavage site

1   GLYALAASPPROSERLEUARGLYSSERGLY
2   VALGLYASNILEPHEILELYSASNLEUASP
3   LYSSERILEASPASNLYSALALEUTYRASP
4   THRPHESERALAPHEGLYASNILELEUSER
5   CYSLYSVALVALCYSASPGLUASNGLYSER
6   LYSGLYTYRGLYPHEVALHISPHEGLUTHR
7   GLNGLUALAALAGLUARGALAILEGLULYS
8   METASNGLYMETLEULEUASNASPARGLYS
9   VALPHEVALGLYARGPHELYSSERARGLYS
10   GLUARGGLUALAGLU

Samples:

sample_1: PABP1, [U-98% 13C; U-98% 15N], 1 mM; sodium chloride 20 mM; sodium phosphate 20 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.04 M; pH: 6.1; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB KFP47809

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts