BMRB Entry 15957
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15957
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Title: Solution structure of the HIV-1 p6 C-terminal domain PubMed: 19254034
Deposition date: 2008-09-18 Original release date: 2009-04-02
Authors: Salgado, Gilmar; Marquant, Rodrigue; Vogel, Alex; Alvez, Isabel; Feller, Scott; Morellet, Nelly; Bouaziz, Serge
Citation: Salgado, Gilmar; Marquant, Rodrigue; Vogel, Alexander; Alves, Isabel; Feller, Scott; Morellet, Nelly; Bouaziz, Serge. "Structural Studies of HIV-1 Gag p6ct and Its Interaction with Vpr Determined by Solution Nuclear Magnetic Resonance" Biochemistry 48, 2355-2367 (2009).
Assembly members:
HIV-1_p6_C-terminal, polymer, 21 residues, 2312.555 Da.
Natural source: Common Name: HIV-1 Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-1
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
HIV-1_p6_C-terminal: DKELYPLASLRSLFGSDPSS
Q
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 146 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HIV-1_p6_C-terminal | 1 |
Entities:
Entity 1, HIV-1_p6_C-terminal 21 residues - 2312.555 Da.
| 1 | ASP | LYS | GLU | LEU | TYR | PRO | LEU | ALA | SER | LEU | ||||
| 2 | ARG | SER | LEU | PHE | GLY | SER | ASP | PRO | SER | SER | ||||
| 3 | GLN |
Samples:
sample_1: HIV-1 p6 C-terminal 2 mM; NaCl 30 mM
2_mM_in_H2O_at_pH_3.3_-_200_eq._DPC: ionic strength: 30 mM; pH: 3.4; pressure: 1 atm; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | 2_mM_in_H2O_at_pH_3.3_-_200_eq._DPC |
| 2D DQF-COSY | sample_1 | isotropic | 2_mM_in_H2O_at_pH_3.3_-_200_eq._DPC |
| 2D 1H-1H NOESY | sample_1 | isotropic | 2_mM_in_H2O_at_pH_3.3_-_200_eq._DPC |
Software:
X-PLOR_NIH_2.16, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz