BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15959

Title: Backbone 1H and 15N Chemical Shift Assignments for the Phosphotyrosine Binding Domain of Insulin Receptor Substrate 1 in the Apo and Phosphopeptide Bound Forms   PubMed: 19053277

Deposition date: 2008-09-18 Original release date: 2008-10-03

Authors: Stone, Martin; Jarymowycz, Virginia

Citation: Stone, Martin; Jarymowycz, Virginia. "Remote Changes in Dynamics of the Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1 Induced by Phosphopeptide Binding"  Biochemistry 47, 13371-13382 (2008).

Assembly members:
Phosphotyrosine-Binding_Domain_of_Insulin_Receptor_Substrate-1, polymer, 111 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):
Phosphotyrosine-Binding_Domain_of_Insulin_Receptor_Substrate-1: GPAFKEVWQVILKPKGLGQT KNLIGIYRLCLTSKTISFVK LNSEAAAVVLQLMNIRRCGH SENFFFIEVGRSAVTGPGEF WMQVDDSVVAQNMHETILEA MRAMSDEFRPR

Data sets:
Data typeCount
15N chemical shifts107
1H chemical shifts107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Monomer1

Entities:

Entity 1, Monomer 111 residues - Formula weight is not available

1   GLYPROALAPHELYSGLUVALTRPGLNVAL
2   ILELEULYSPROLYSGLYLEUGLYGLNTHR
3   LYSASNLEUILEGLYILETYRARGLEUCYS
4   LEUTHRSERLYSTHRILESERPHEVALLYS
5   LEUASNSERGLUALAALAALAVALVALLEU
6   GLNLEUMETASNILEARGARGCYSGLYHIS
7   SERGLUASNPHEPHEPHEILEGLUVALGLY
8   ARGSERALAVALTHRGLYPROGLYGLUPHE
9   TRPMETGLNVALASPASPSERVALVALALA
10   GLNASNMETHISGLUTHRILELEUGLUALA
11   METARGALAMETSERASPGLUPHEARGPRO
12   ARG

Samples:

PTB_Domain_of_IRS-1_Apo_State: Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1, [U-95% 13C], 200 uM

PTB_Domain_of_IRS-1_Apo_Sample_Conditions: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBPTB_Domain_of_IRS-1_Apo_StateisotropicPTB_Domain_of_IRS-1_Apo_Sample_Conditions
3D CBCA(CO)NHPTB_Domain_of_IRS-1_Apo_StateisotropicPTB_Domain_of_IRS-1_Apo_Sample_Conditions

Software:

FELIX, Accelrys Software Inc. - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA11026 BAC32308 BAE24991 BAG10207
EMBL CAA41264 CAA49378
GB AAA39335 AAB21608 AAB27175 AAH53895 AAI56077
PRF 1712323A
REF NP_001231418 NP_005535 NP_034700 NP_037101 XP_001109882
SP P35568 P35569 P35570 Q28224
TPG DAA32358
AlphaFold P35568 P35569 P35570 Q28224

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts