BMRB Entry 15962
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15962
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Citation: Alontaga, Aileen; Rodriguez, Juan; Schonbrunn, Ernst; Becker, Andreas; Funke, Todd; Yukl, Erik; Hayashi, Takahiro; Stobaugh, Jordan; Moenne-Loccoz, Pierre; Rivera, Mario. "Structural characterization of the hemophore HasAp from Pseudomonas aeruginosa:
NMR spectroscopy reveals protein-protein interactions between Holo-HasAp and
hemoglobin." Biochemistry 48, 96-109 (2009).
PubMed: 19072037
Assembly members:
HasAp_truncated_polypeptide, polymer, 184 residues, 19000 Da.
HEM, non-polymer, 616.487 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 11a
Entity Sequences (FASTA):
HasAp_truncated_polypeptide: MSISISYSTTYSGWTVADYL
ADWSAYFGDVNHRPGQVVDG
SNTGGFNPGPFDGSQYALKS
TASDAAFIAGGDLHYTLFSN
PSHTLWGKLDSIALGDTLTG
GASSGGYALDSQEVSFSNLG
LDSPIAQGRDGTVHKVVYGL
MSGDSSALQGQIDALLKAVD
PSLSINSTFDQLAAAGVAHA
TPAA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 518 |
| 15N chemical shifts | 170 |
| 1H chemical shifts | 170 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | monomer | 1 |
| 2 | cofactor | 2 |
Entities:
Entity 1, monomer 184 residues - 19000 Da.
| 1 | MET | SER | ILE | SER | ILE | SER | TYR | SER | THR | THR | ||||
| 2 | TYR | SER | GLY | TRP | THR | VAL | ALA | ASP | TYR | LEU | ||||
| 3 | ALA | ASP | TRP | SER | ALA | TYR | PHE | GLY | ASP | VAL | ||||
| 4 | ASN | HIS | ARG | PRO | GLY | GLN | VAL | VAL | ASP | GLY | ||||
| 5 | SER | ASN | THR | GLY | GLY | PHE | ASN | PRO | GLY | PRO | ||||
| 6 | PHE | ASP | GLY | SER | GLN | TYR | ALA | LEU | LYS | SER | ||||
| 7 | THR | ALA | SER | ASP | ALA | ALA | PHE | ILE | ALA | GLY | ||||
| 8 | GLY | ASP | LEU | HIS | TYR | THR | LEU | PHE | SER | ASN | ||||
| 9 | PRO | SER | HIS | THR | LEU | TRP | GLY | LYS | LEU | ASP | ||||
| 10 | SER | ILE | ALA | LEU | GLY | ASP | THR | LEU | THR | GLY | ||||
| 11 | GLY | ALA | SER | SER | GLY | GLY | TYR | ALA | LEU | ASP | ||||
| 12 | SER | GLN | GLU | VAL | SER | PHE | SER | ASN | LEU | GLY | ||||
| 13 | LEU | ASP | SER | PRO | ILE | ALA | GLN | GLY | ARG | ASP | ||||
| 14 | GLY | THR | VAL | HIS | LYS | VAL | VAL | TYR | GLY | LEU | ||||
| 15 | MET | SER | GLY | ASP | SER | SER | ALA | LEU | GLN | GLY | ||||
| 16 | GLN | ILE | ASP | ALA | LEU | LEU | LYS | ALA | VAL | ASP | ||||
| 17 | PRO | SER | LEU | SER | ILE | ASN | SER | THR | PHE | ASP | ||||
| 18 | GLN | LEU | ALA | ALA | ALA | GLY | VAL | ALA | HIS | ALA | ||||
| 19 | THR | PRO | ALA | ALA |
Entity 2, cofactor - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Related Database Links:
| BMRB | 15963 19720 19721 19722 |
| PDB | 3ELL 3MOK 3MOL 3MOM 3W8M 3W8O 3WAH 4O6Q 4O6S 4O6T 4O6U |
| DBJ | BAK91367 BAP21966 BAP49596 BAQ38483 BAR66548 |
| EMBL | CAA76520 CAW26382 CCQ88896 CDH69922 CDH76002 |
| GB | AAG06795 AAT49927 ABJ12662 ABR84528 AEO74046 |
| REF | NP_252097 WP_003091842 WP_003115011 WP_003124283 WP_003153113 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated peaks