BMRB Entry 16009
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16009
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Citation: Heinamaki, Kimmo; Oeemig, Jesper; Djupsjobacka, Janica; Iwai, Hideo. "NMR resonance assignment of DnaE intein from Nostoc punctiforme" Biomol. NMR Assignments 3, 41-43 (2009).
PubMed: 19636943
Assembly members:
intein_polypeptide, polymer, 139 residues, Formula weight is not available
Natural source: Common Name: cyanobacterium Taxonomy ID: 272131 Superkingdom: Bacteria Kingdom: not available Genus/species: Nostoc Nostoc punctiforme
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHYRSF53
Entity Sequences (FASTA):
intein_polypeptide: GGALSYETEILTVEYGLLPI
GKIVEKRIECTVYSVDNNGN
IYTQPVAQWHDRGEQEVFEY
CLEDGSLIRATKDHKFMTVD
GQMLPIDEIFERELDLMRVD
NLPNIKIATRKYLGKQNVYD
IGVERDHNFALKNGFIASN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 611 |
| 15N chemical shifts | 146 |
| 1H chemical shifts | 969 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | single chain intein | 1 |
Entities:
Entity 1, single chain intein 139 residues - Formula weight is not available
| 1 | GLY | GLY | ALA | LEU | SER | TYR | GLU | THR | GLU | ILE | ||||
| 2 | LEU | THR | VAL | GLU | TYR | GLY | LEU | LEU | PRO | ILE | ||||
| 3 | GLY | LYS | ILE | VAL | GLU | LYS | ARG | ILE | GLU | CYS | ||||
| 4 | THR | VAL | TYR | SER | VAL | ASP | ASN | ASN | GLY | ASN | ||||
| 5 | ILE | TYR | THR | GLN | PRO | VAL | ALA | GLN | TRP | HIS | ||||
| 6 | ASP | ARG | GLY | GLU | GLN | GLU | VAL | PHE | GLU | TYR | ||||
| 7 | CYS | LEU | GLU | ASP | GLY | SER | LEU | ILE | ARG | ALA | ||||
| 8 | THR | LYS | ASP | HIS | LYS | PHE | MET | THR | VAL | ASP | ||||
| 9 | GLY | GLN | MET | LEU | PRO | ILE | ASP | GLU | ILE | PHE | ||||
| 10 | GLU | ARG | GLU | LEU | ASP | LEU | MET | ARG | VAL | ASP | ||||
| 11 | ASN | LEU | PRO | ASN | ILE | LYS | ILE | ALA | THR | ARG | ||||
| 12 | LYS | TYR | LEU | GLY | LYS | GLN | ASN | VAL | TYR | ASP | ||||
| 13 | ILE | GLY | VAL | GLU | ARG | ASP | HIS | ASN | PHE | ALA | ||||
| 14 | LEU | LYS | ASN | GLY | PHE | ILE | ALA | SER | ASN |
Related Database Links:
| PDB | 2KEQ 4KL5 4QFQ |
| GB | ACC83218 ADG44968 ADG44969 AIS22787 AIS22800 |
| REF | WP_012411174 |
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