BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16021

Title: NMR assignments of juvenile hormone binding protein in complex with JH III   PubMed: 19636950

Deposition date: 2008-11-05 Original release date: 2009-01-28

Authors: Tase, Akira; Fujimoto, Zui; Shiotsuki, Takahiro

Citation: Suzuki, Rintaro; Tase, Akira; Fujimoto, Zui; Shiotsuki, Takahiro; Yamazaki, Toshimasa. "NMR assignments of juvenile hormone binding protein in complex with JH III"  Biomol. NMR Assignments 3, 73-76 (2009).

Assembly members:
hJHBP, polymer, 227 residues, Formula weight is not available

Natural source:   Common Name: Silkworm   Taxonomy ID: 7091   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bombyx mori

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Entity Sequences (FASTA):
hJHBP: GSDGDALLKPCKLGDMQCLS SATEQFLEKTSKGIPQYDIW PIDPLVVTSLDVIAPSDAGI VIRFKNLNITGLKNQQISDF QMDTKAKTVLLKTKADLHIV GDIVIELTEQSKSFTGLYTA DTNVIGAVRYGYNLKNDDNG VQHFEVQPETFTCESIGEPK ITLSSDLSSALEKDSGNNSL EPDMEPLKTLRQAAICKIAE ACYISVVHNIRASAKILPAS SFFENLN

Data sets:
Data typeCount
13C chemical shifts1048
15N chemical shifts242
1H chemical shifts1601

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hJHBP1

Entities:

Entity 1, hJHBP 227 residues - Formula weight is not available

Residues (-2)-(-1) represent a non-native sequence from affinity tag.

1   GLYSERASPGLYASPALALEULEULYSPRO
2   CYSLYSLEUGLYASPMETGLNCYSLEUSER
3   SERALATHRGLUGLNPHELEUGLULYSTHR
4   SERLYSGLYILEPROGLNTYRASPILETRP
5   PROILEASPPROLEUVALVALTHRSERLEU
6   ASPVALILEALAPROSERASPALAGLYILE
7   VALILEARGPHELYSASNLEUASNILETHR
8   GLYLEULYSASNGLNGLNILESERASPPHE
9   GLNMETASPTHRLYSALALYSTHRVALLEU
10   LEULYSTHRLYSALAASPLEUHISILEVAL
11   GLYASPILEVALILEGLULEUTHRGLUGLN
12   SERLYSSERPHETHRGLYLEUTYRTHRALA
13   ASPTHRASNVALILEGLYALAVALARGTYR
14   GLYTYRASNLEULYSASNASPASPASNGLY
15   VALGLNHISPHEGLUVALGLNPROGLUTHR
16   PHETHRCYSGLUSERILEGLYGLUPROLYS
17   ILETHRLEUSERSERASPLEUSERSERALA
18   LEUGLULYSASPSERGLYASNASNSERLEU
19   GLUPROASPMETGLUPROLEULYSTHRLEU
20   ARGGLNALAALAILECYSLYSILEALAGLU
21   ALACYSTYRILESERVALVALHISASNILE
22   ARGALASERALALYSILELEUPROALASER
23   SERPHEPHEGLUASNLEUASN

Samples:

15N: hJHBP, [U-15N], 0.6-1.0 mM; JH III 0.6-1.0 mM; sodium phosphate 45 mM; H2O 90%; D2O 10%

13C_15N: hJHBP, [U-13C; U-15N], 0.6-1.0 mM; JH III 0.6-1.0 mM; sodium phosphate 45 mM; H2O 90%; D2O 10%

13C_15N_in_100%_D2O: hJHBP, [U-13C; U-15N], 0.6-1.0 mM; JH III 0.6-1.0 mM; sodium phosphate 45 mM; D2O 100%

pH_6.0_35C: pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15NisotropicpH_6.0_35C
2D 1H-13C HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D HNCO13C_15NisotropicpH_6.0_35C
3D CBCA(CO)NH13C_15NisotropicpH_6.0_35C
3D HNCA13C_15NisotropicpH_6.0_35C
3D HCABGCO13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D HCCH-TOCSY13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D 15N-separated HOHAHA-HSQC15NisotropicpH_6.0_35C
3D 15N-separated NOESY-HSQC15NisotropicpH_6.0_35C
3D 13C/15N-separated NOESY-HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D 13C-separated NOESY-HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D 13C/13C-separated NOESY-HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C

Software:

xwinnmr v3.1, Bruker Biospin - collection

NMRPipe vreleased at Feb 10, 2006, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

GB AF098304 AAF19267 AAF19268 AAP41112
SWS Q9U556_BOMMO
BMRB 11073
PDB
REF NP_001037074

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts