BMRB Entry 16041
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16041
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Title: FLNCCPGCCMEP_ReAsH_motif PubMed: 19281235
Deposition date: 2008-11-24 Original release date: 2009-03-23
Authors: Madani, Fatemeh; Lind, Jesper; Damberg, Peter; Adams, Stephen; Tsien, Roger; Graslund, Astrid
Citation: Madani, Fatemeh; Lind, Jesper; Damberg, Peter; Adams, Stephen; Tsien, Roger; Graslund, Astrid. "Hairpin Structure of a Biarsenical-Tetracysteine Motif Determined by NMR" J. Am. Chem. Soc. 131, 4613-4615 (2009).
Assembly members:
FLNCCPGCCMEP, polymer, 12 residues, Formula weight is not available
ReAsH, non-polymer, 360 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
FLNCCPGCCMEP: FLNCCPGCCMEP
- assigned_chemical_shifts
- conformer_family_coord_set
Data type | Count |
1H chemical shifts | 66 |
conformer coordinate sets | 1 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | FLNCCPGCCMEP | 1 |
2 | ReAsH, resorufin arsenical hairpin binder | 2 |
Entities:
Entity 1, FLNCCPGCCMEP 12 residues - Formula weight is not available
1 | PHE | LEU | ASN | CYS | CYS | PRO | GLY | CYS | CYS | MET | ||||
2 | GLU | PRO |
Entity 2, ReAsH, resorufin arsenical hairpin binder - C12 H4 N O3 As2 - 360 Da.
Samples:
sample_1: FLNCCPGCCMEP 0.6 mM
sample_conditions_1: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 278 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - refinement
NMR spectrometers:
- Bruker Avance 500 MHz
- Varian INOVA 600 MHz