BMRB Entry 16097

Assembly:

Entities:

Entity 1, CtR107 166 residues - Formula weight is not available

Samples:

NC: CtR107, [U-99% 13C; U-99% 15N], 0.45 mM; D2O, [U-2H], 5%; H2O 95%; DSS 50 uM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM; sodium chloride 100 mM; MES 20 mM

NC5: CtR107, [U-5% 13C; U-99% 15N], 0.45 mM; D2O, [U-2H], 5%; H2O 95%; DSS 50 uM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM; sodium chloride 100 mM; MES 20 mM

sample_conditions_1: ionic strength: 235 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPSCAN, Glaser - processing

XEASY, Bartels et al. - chemical shift assignment

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

CSI, (CSI) Wishart and Sykes - structure solution

TALOS, Cornilescu, Delaglio and Bax - structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization

Molmol, Koradi, Billeter and Wuthrich - data analysis

PSVS, Bhattacharya and Montelione - refinement

NMR spectrometers:

• Varian INOVA 600 MHz
• Varian INOVA 750 MHz