BMRB Entry 16115
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16115
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Title: Solution Structure of an SH3-like Domain from p80-Coilin PubMed: 20875822
Deposition date: 2009-01-10 Original release date: 2011-06-01
Authors: Donaldson, Logan
Citation: Shanbhag, Riya; Kurabi, Arwa; Kwan, Jamie; Donaldson, Logan. "Solution structure of the carboxy-terminal Tudor domain from human Coilin." FEBS Lett. 584, 4351-4356 (2010).
Assembly members:
coilin, polymer, 141 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
coilin: MGSSHHHHHHSSGLVPRGSH
GSHMDYSLLPLLAAAPQVGE
KIAFKLLELTSSYSPDVSDY
KEGRILSHNPETQQVDIEIL
SSLPALREPGKFDLVYHNEN
GAEVVEYAVTQESKITVFWK
ELIDPRLIIESPSNTSSTEP
A
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 323 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 465 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | coilin | 1 |
Entities:
Entity 1, coilin 141 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | GLY | SER | HIS | MET | ASP | TYR | SER | LEU | LEU | PRO | ||||
| 4 | LEU | LEU | ALA | ALA | ALA | PRO | GLN | VAL | GLY | GLU | ||||
| 5 | LYS | ILE | ALA | PHE | LYS | LEU | LEU | GLU | LEU | THR | ||||
| 6 | SER | SER | TYR | SER | PRO | ASP | VAL | SER | ASP | TYR | ||||
| 7 | LYS | GLU | GLY | ARG | ILE | LEU | SER | HIS | ASN | PRO | ||||
| 8 | GLU | THR | GLN | GLN | VAL | ASP | ILE | GLU | ILE | LEU | ||||
| 9 | SER | SER | LEU | PRO | ALA | LEU | ARG | GLU | PRO | GLY | ||||
| 10 | LYS | PHE | ASP | LEU | VAL | TYR | HIS | ASN | GLU | ASN | ||||
| 11 | GLY | ALA | GLU | VAL | VAL | GLU | TYR | ALA | VAL | THR | ||||
| 12 | GLN | GLU | SER | LYS | ILE | THR | VAL | PHE | TRP | LYS | ||||
| 13 | GLU | LEU | ILE | ASP | PRO | ARG | LEU | ILE | ILE | GLU | ||||
| 14 | SER | PRO | SER | ASN | THR | SER | SER | THR | GLU | PRO | ||||
| 15 | ALA |
Samples:
sample: sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.05%; coilin; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 150 mM; pH: 7.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D simultaneous 1H-13C-15N NOESY | sample | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample | isotropic | sample_conditions_1 |
| 3D HNCO | sample | isotropic | sample_conditions_1 |
| 3D HNCACB | sample | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample | isotropic | sample_conditions_1 |
| 3D aromatic 1H-13C NOESY | sample | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMRView, Johnson, One Moon Scientific - peak picking
TALOS, Cornilescu, Delaglio and Bax - dihedral restraints
NMR spectrometers:
- Varian NMRS 600 MHz
Related Database Links:
| DBJ | BAE01330 BAG36378 |
| GB | AAA36412 AAB81550 AAH10385 ABM83853 ABM87175 |
| REF | NP_004636 XP_002827396 XP_009431163 XP_511900 |
| SP | P38432 |
| AlphaFold | P38432 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts