BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16120

Title: Sequence-specific 1H, 13C, and 15N resonance assignment of the Autophagy-related Protein Atg 8   PubMed: 19636965

Deposition date: 2009-01-14 Original release date: 2009-04-14

Authors: Schwarten, Melanie; Stoldt, Matthias; Mohrluder, Jeannine; Willbold, Dieter

Citation: Schwarten, Melanie; Stoldt, Matthias; Mohrluder, Jeannine; Willbold, Dieter. "Sequence-specific 1H, 13C, and 15N resonance assignment of the autophagy-related protein Atg8"  Biomol. NMR Assignments 3, 137-139 (2009).

Assembly members:
Atg8, polymer, 119 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex-4T-2

Entity Sequences (FASTA):
Atg8: GSMKSTFKSEYPFEKRKAES ERIADRFKNRIPVICEKAEK SDIPEIDKRKYLVPADLTVG QFVYVIRKRIMLPPEKAIFI FVNDTLPPTAALMSAIYQEH KDKDGFLYVTYSGENTFGR

Data sets:
Data typeCount
13C chemical shifts475
15N chemical shifts106
1H chemical shifts736

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Atg81

Entities:

Entity 1, Atg8 119 residues - Formula weight is not available

1   GLYSERMETLYSSERTHRPHELYSSERGLU
2   TYRPROPHEGLULYSARGLYSALAGLUSER
3   GLUARGILEALAASPARGPHELYSASNARG
4   ILEPROVALILECYSGLULYSALAGLULYS
5   SERASPILEPROGLUILEASPLYSARGLYS
6   TYRLEUVALPROALAASPLEUTHRVALGLY
7   GLNPHEVALTYRVALILEARGLYSARGILE
8   METLEUPROPROGLULYSALAILEPHEILE
9   PHEVALASNASPTHRLEUPROPROTHRALA
10   ALALEUMETSERALAILETYRGLNGLUHIS
11   LYSASPLYSASPGLYPHELEUTYRVALTHR
12   TYRSERGLYGLUASNTHRPHEGLYARG

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 150 mM; DTT 5 mM; EDTA 1 mM; sodium azide 0.05%; Atg8, [U-13C; U-15N], 0.5 mM

sample_2: sodium phosphate 20 mM; sodium chloride 150 mM; DTT 5 mM; EDTA 1 mM; sodium azide 0.05%; Atg8, [U-13C; U-15N], 0.5 mM; DSS 1 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
1D 1Hsample_2isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller - chemical shift assignment

NMR spectrometers:

  • Varian VNMRS 800 MHz
  • Varian Unity_INOVA 600 MHz

Related Database Links:

BMRB 16835 17879
PDB
DBJ GAA21477
EMBL CAA56032 CAA84899 CAY77706
GB AAT92889 AHY74413 AJP37015 AJP81917 AJP82297
REF NP_009475
SP A6ZKM4 P38182
TPG DAA07045
AlphaFold P38182 A6ZKM4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts