BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16133

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for TolAIII residues 296-421   PubMed: 19306883

Deposition date: 2009-01-23 Original release date: 2009-04-22

Authors: Hecht, Oliver

Citation: Hecht, Oliver; Ridley, Helen; Lakey, Jeremy; Moore, Geoffrey. "A common interaction for the entry of colicin N and filamentous phage into Escherichia coli"  J. Mol. Biol. 388, 880-893 (2009).

Assembly members:
TolAIII, polymer, 135 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet8c

Entity Sequences (FASTA):
TolAIII: HHHHHHSSQIFSVTLSSGKN APKTGGGAKGNNASPAGSGN TKNNGASGADINNYAGQIKS AIESKFYDASSYAGKTCTLR IKLAPDGMLLDIKPEGGDPA LCQAALAAAKLAKIPKPPSQ AVYEVFKNAPLDFKP

Data sets:
Data typeCount
13C chemical shifts145
15N chemical shifts77
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TolAIII1

Entities:

Entity 1, TolAIII 135 residues - Formula weight is not available

1   HISHISHISHISHISHISSERSERGLNILE
2   PHESERVALTHRLEUSERSERGLYLYSASN
3   ALAPROLYSTHRGLYGLYGLYALALYSGLY
4   ASNASNALASERPROALAGLYSERGLYASN
5   THRLYSASNASNGLYALASERGLYALAASP
6   ILEASNASNTYRALAGLYGLNILELYSSER
7   ALAILEGLUSERLYSPHETYRASPALASER
8   SERTYRALAGLYLYSTHRCYSTHRLEUARG
9   ILELYSLEUALAPROASPGLYMETLEULEU
10   ASPILELYSPROGLUGLYGLYASPPROALA
11   LEUCYSGLNALAALALEUALAALAALALYS
12   LEUALALYSILEPROLYSPROPROSERGLN
13   ALAVALTYRGLUVALPHELYSASNALAPRO
14   LEUASPPHELYSPRO

Samples:

sample_1: TolAIII, [U-100% 13C; U-100% 15N], 0.6 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.35 M; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16134
PDB
EMBL CTW32328
GB AHA63696 AHG07456 AHY69283 EGB67246 EGC08283
REF WP_000030617 WP_000030621 WP_000030643 WP_000030697 WP_000030705

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts