BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16162

Title: Beta1D Integrin Cytoplasmic Tail (Polyhistidine-Tagged) 1H and 15N Chemical Shift Assignments   PubMed: 19798053

Deposition date: 2009-02-07 Original release date: 2009-10-07

Authors: Anthis, Nicholas; Wegener, Kate; Campbell, Iain

Citation: Anthis, Nicholas; Wegener, Kate; Ye, Feng; Kim, Chungho; Goult, Benjamin; Lowe, Edward; Vakonakis, Ioannis; Bate, Neil; Critchley, David; Ginsberg, Mark; Campbell, Iain. "The structure of an integrin/talin complex reveals the basis of inside-out signal transduction."  EMBO J. 28, 3623-3632 (2009).

Assembly members:
beta1D_integrin_with_C-terminal_tag, polymer, 60 residues, 7251 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30b

Entity Sequences (FASTA):
beta1D_integrin_with_C-terminal_tag: MKLLMIIHDRREFAKFEKEK MNAKWDTQENPIYKSPINNF KNPNYGRKAGLGSEHHHHHH

Data sets:
Data typeCount
15N chemical shifts118
1H chemical shifts392

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta1D integrin tail1

Entities:

Entity 1, beta1D integrin tail 60 residues - 7251 Da.

This is the cytoplasmic tail of the beta1D integrin tail. The first residue (M) and the last nine residues (GSEHHHHHH) are cloning artifacts.

1   METLYSLEULEUMETILEILEHISASPARG
2   ARGGLUPHEALALYSPHEGLULYSGLULYS
3   METASNALALYSTRPASPTHRGLNGLUASN
4   PROILETYRLYSSERPROILEASNASNPHE
5   LYSASNPROASNTYRGLYARGLYSALAGLY
6   LEUGLYSERGLUHISHISHISHISHISHIS

Samples:

pH_6.1: D2O 5%; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; DSS 0.1 mM; beta1D integrin with C-terminal tag, [U-15N], 0.05 mM; H2O 95%

pH_5.0: D2O 5%; sodium acetate 20 mM; sodium azide 0.02%; DSS 0.1 mM; beta1D integrin with C-terminal tag, [U-15N], 1 mM; D2O 5%

pH_6.1_conditions: ionic strength: 200 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K

pH_5.0_conditions: ionic strength: 10 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCpH_6.1isotropicpH_6.1_conditions
3D 1H-15N NOESYpH_5.0isotropicpH_5.0_conditions
3D 1H-15N TOCSYpH_5.0isotropicpH_5.0_conditions
2D 1H-15N HSQCpH_5.0isotropicpH_5.0_conditions

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

Omega Spectrometer Operating Software vBeta 6.0.3b2, GE - collection

ANALYSIS, Boucher and Stevens - chemical shift assignment

NMR spectrometers:

  • GE OMEGA 600 MHz
  • GE OMEGA 500 MHz

Related Database Links:

BMRB 16158
PDB
GB AAA79833 AAA80243 AAA81366 AAA88821 AAI14108
PIR S66396
REF NP_001288146 NP_391988 XP_003364388 XP_004387928 XP_004401881

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts