BMRB Entry 16162

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16162

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Title:
Beta1D Integrin Cytoplasmic Tail (Polyhistidine-Tagged) 1H and 15N Chemical Shift Assignments
Deposition date:
2009-02-07
Original release date:
2009-10-07
Authors:
Anthis, Nicholas; Wegener, Kate; Campbell, Iain
Citation:

Citation: Anthis, Nicholas; Wegener, Kate; Ye, Feng; Kim, Chungho; Goult, Benjamin; Lowe, Edward; Vakonakis, Ioannis; Bate, Neil; Critchley, David; Ginsberg, Mark; Campbell, Iain. "The structure of an integrin/talin complex reveals the basis of inside-out signal transduction."  EMBO J. 28, 3623-3632 (2009).
PubMed: 19798053

Assembly members:

Assembly members:
beta1D_integrin_with_C-terminal_tag, polymer, 60 residues, 7251 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta1D_integrin_with_C-terminal_tag: MKLLMIIHDRREFAKFEKEK MNAKWDTQENPIYKSPINNF KNPNYGRKAGLGSEHHHHHH

Data sets:
Data typeCount
15N chemical shifts118
1H chemical shifts392

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1beta1D integrin tail1

Entities:

Entity 1, beta1D integrin tail 60 residues - 7251 Da.

This is the cytoplasmic tail of the beta1D integrin tail. The first residue (M) and the last nine residues (GSEHHHHHH) are cloning artifacts.

1   METLYSLEULEUMETILEILEHISASPARG
2   ARGGLUPHEALALYSPHEGLULYSGLULYS
3   METASNALALYSTRPASPTHRGLNGLUASN
4   PROILETYRLYSSERPROILEASNASNPHE
5   LYSASNPROASNTYRGLYARGLYSALAGLY
6   LEUGLYSERGLUHISHISHISHISHISHIS

Related Database Links:

BMRB 16158
PDB 3G9W
GB AAA79833 AAA80243 AAA81366 AAA88821 AAI14108
PIR S66396
REF NP_001288146 NP_391988 XP_003364388 XP_004387928 XP_004401881

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks