BMRB Entry 16294
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16294
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Title: 1H (NH, HA, HB, other) Chemical Shift Assignments of Peach Pru p 3, Non-Specific Lipid Transfer Protein (nsLTP) isolated from Peach fruit skin PubMed: 20121231
Deposition date: 2009-05-08 Original release date: 2010-03-01
Authors: Wijesinha-Bettoni, Ramani; Alexeev, Yuri; Johnson, Phil; Marsh, Justin; Sancho, Ana; Mackie, Alan; Shewry, Peter; Smith, Lorna; Mills, E. N
Citation: Wijesinha-Bettoni, Ramani; Alexeev, Yuri; Johnson, Phil; Marsh, Justin; Sancho, Ana; Abdullah, Syed; Mackie, Alan; Shewry, Peter; Smith, Lorna; Mills, E. N Clare. "The structural characteristics of nonspecific lipid transfer proteins explain their resistance to gastroduodenal proteolysis." Biochemistry 49, 2130-2139 (2010).
Assembly members:
Pru_p_3_(nsLTP), polymer, 91 residues, Formula weight is not available
Natural source: Common Name: peach Taxonomy ID: 3760 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Prunus persica
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Pru_p_3_(nsLTP): ITCGQVSSSLAPCIPYVRGG
GAVPPACCNGIRNVNNLART
TPDRQAACNCLKQLSASVPG
VNPNNAAALPGKCGVSIPYK
ISASTNCATVK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 332 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | non specific lipid transfer protein from peach (Pru p 3) | 1 |
Entities:
Entity 1, non specific lipid transfer protein from peach (Pru p 3) 91 residues - Formula weight is not available
| 1 | ILE | THR | CYS | GLY | GLN | VAL | SER | SER | SER | LEU | ||||
| 2 | ALA | PRO | CYS | ILE | PRO | TYR | VAL | ARG | GLY | GLY | ||||
| 3 | GLY | ALA | VAL | PRO | PRO | ALA | CYS | CYS | ASN | GLY | ||||
| 4 | ILE | ARG | ASN | VAL | ASN | ASN | LEU | ALA | ARG | THR | ||||
| 5 | THR | PRO | ASP | ARG | GLN | ALA | ALA | CYS | ASN | CYS | ||||
| 6 | LEU | LYS | GLN | LEU | SER | ALA | SER | VAL | PRO | GLY | ||||
| 7 | VAL | ASN | PRO | ASN | ASN | ALA | ALA | ALA | LEU | PRO | ||||
| 8 | GLY | LYS | CYS | GLY | VAL | SER | ILE | PRO | TYR | LYS | ||||
| 9 | ILE | SER | ALA | SER | THR | ASN | CYS | ALA | THR | VAL | ||||
| 10 | LYS |
Samples:
sample_1: Pru p 3 (nsLTP)2.0 – 3.0 mM; H2O 95%; D2O 5%
sample_conditions_1: pH: 5.3; temperature: 310 K
sample_conditions_2: pH: 5.3; temperature: 298 K
sample_conditions_3: pH: 4; temperature: 298 K
sample_conditions_4: pH: 6.5; temperature: 293 K
sample_conditions_5: pH: 1.8; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_3 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_4 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_4 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_4 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_5 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_5 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_5 |
Software:
SPARKY vVersion 3.110, Goddard - data analysis
NMR spectrometers:
- Home-built ?,AM 600 MHz