BMRB Entry 16341

Name: The inactive form of the retroviral protease of the murine intracisternal particle, inMIA-14 PR
Published: 2009-11-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16341

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: The inactive form of the retroviral protease of the murine intracisternal particle, inMIA-14 PR PubMed: 19856131

Deposition date: 2009-06-09 Original release date: 2009-11-16

Authors: Motackova, Veronika; Kubickova, Monika; Kozisek, Milan; Grantz-Saskova, Klara; Svec, Martin; Zidek, Lukas; Sklenar, Vladimir

Citation: Motakova, Veronika; Kubikova, Monika; Kozisek, Milan; Sakova, Klara; Svec, Martin; Zidek, Luka; Sklena, Vladimir. "Backbone (1)H, (13)C, and (15)N NMR assignment for the inactive form of the retroviral protease of the murine intracisternal A-type particle, inMIA-14 PR." Biomol NMR Assign 3, 261-264 (2009).

Assembly members:
inMIA14-PR, polymer, 156 residues, Formula weight is not available

Natural source: Common Name: Murine endogenous retrovirus Taxonomy ID: 35275 Superkingdom: Viruses Kingdom: not available Genus/species: Betaretrovirus Murine endogenous retrovirus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22

Entity Sequences (FASTA):
inMIA14-PR: LVVSLNDRPKLRLKINGKEF EGILDTGADKSIISTHWWPK AWPTTESSHSLQGLGYQSCP TISSVALTWESSEGQQGKFI PYVLPLPVNLWGRDIMQHLG LILSNENAPSGGYSTKAKNI MAKMGYKEGKGLGHQEQGRI EPISPNGNQDRQGLGF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	416
15N chemical shifts	135
1H chemical shifts	135

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	inMIA14-PR	1

Entities:

Entity 1, inMIA14-PR 156 residues - Formula weight is not available

1

LEU

VAL

SER

LEU

ASN

ASP

ARG

PRO

LYS

2

LEU

ARG

LEU

LYS

ILE

ASN

GLY

LYS

GLU

PHE

3

GLU

GLY

ILE

LEU

ASP

THR

GLY

ALA

ASP

LYS

4

SER

ILE

SER

THR

HIS

TRP

PRO

LYS

5

ALA

TRP

PRO

THR

GLU

SER

HIS

SER

6

LEU

GLN

GLY

LEU

GLY

TYR

GLN

SER

CYS

PRO

7

THR

ILE

SER

VAL

ALA

LEU

THR

TRP

GLU

8

SER

GLU

GLY

GLN

GLY

LYS

PHE

ILE

9

PRO

TYR

VAL

LEU

PRO

LEU

PRO

VAL

ASN

LEU

10

TRP

GLY

ARG

ASP

ILE

MET

GLN

HIS

LEU

GLY

11

LEU

ILE

LEU

SER

ASN

GLU

ASN

ALA

PRO

SER

12

GLY

TYR

SER

THR

LYS

ALA

LYS

ASN

ILE

13

MET

ALA

LYS

MET

GLY

TYR

LYS

GLU

GLY

LYS

14

GLY

LEU

GLY

HIS

GLN

GLU

GLN

GLY

ARG

ILE

15

GLU

PRO

ILE

SER

PRO

ASN

GLY

ASN

GLN

ASP

16

ARG

GLN

GLY

LEU

GLY

PHE

Samples:

sample_1: inMIA14-PR, [U-100% 13C; U-100% 15N; U-75% 2H], 0.3 mM; sodium chloride 50 mM; sodium azide 50 uM; phosphate buffer 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.4; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

DBJ	BAC79171
EMBL	CRH48730 CRH55731 CRH56783 CRH57961
GB	AAC12789 AAC52922
REF	WP_057247684 WP_057256782
SP	P11365
AlphaFold	P11365

Biological Magnetic Resonance Data Bank