BMRB Entry 16405
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16405
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Title: Solution Structure of the Tandem UIM Domain of Ataxin-3 Complexed with Ubiquitin PubMed: 20949063
Deposition date: 2009-07-12 Original release date: 2012-08-03
Authors: Zhou, Chenjie; Song, Aixin; Lin, Donghai; Hu, Hongyu
Citation: Song, Ai-Xin; Zhou, Chen-Jie; Peng, Yu; Gao, Xue-Chao; Zhou, Zi-Ren; Fu, Qing-Shan; Hong, Jing; Lin, Dong-Hai; Hu, Hong-Yu. "Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains" PLoS ONE 5, e13202-e13202 (2010).
Assembly members:
Ataxin-3/Ubiquitin complex, polymer, 46 residues, 5268.689 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGBTNH
Entity Sequences (FASTA):
Ataxin-3/Ubiquitin complex: GSLDEDEEDLQRALALSRQE
IDMEDEEADLRRAIQLSMQG
SSRNLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 133 |
| 15N chemical shifts | 41 |
| 1H chemical shifts | 241 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ataxin-3/Ubiquitin complex | 1 |
Entities:
Entity 1, Ataxin-3/Ubiquitin complex 46 residues - 5268.689 Da.
| 1 | GLY | SER | LEU | ASP | GLU | ASP | GLU | GLU | ASP | LEU | ||||
| 2 | GLN | ARG | ALA | LEU | ALA | LEU | SER | ARG | GLN | GLU | ||||
| 3 | ILE | ASP | MET | GLU | ASP | GLU | GLU | ALA | ASP | LEU | ||||
| 4 | ARG | ARG | ALA | ILE | GLN | LEU | SER | MET | GLN | GLY | ||||
| 5 | SER | SER | ARG | ASN | LEU | GLU |
Samples:
sample_1: entity, [U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.02%; ubiquitin 4 mM
sample_2: entity, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.02%; ubiquitin 4 mM
sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment, data analysis
ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution
ProcheckNMR, Laskowski and MacArthur - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| GB | ADD00635 EHB02478 ELW55804 |
| REF | XP_004584394 XP_004681665 XP_004681676 XP_004837073 XP_009004709 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts