BMRB Entry 16420
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16420
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Title: Protein stablilization by specific binding of guanidinium to a functional arginine-binding surface on an SH3 domain PubMed: 16373478
Deposition date: 2009-07-23 Original release date: 2015-09-18
Authors: Zarrine-Afsar, Arash; Mittermaier, Anthony; Kay, Lewis; Davidson, Alan
Citation: Zarrine-Afsar, Arash; Mittermaier, Anthony; Kay, Lewis; Davidson, Alan. "Protein stabilization by specific binding of guanidinium to a functional arginine-binding surface on an SH3 domain" Protein Sci. 1, 162-170 (2006).
Assembly members:
Fyn_SH3_domain, polymer, 59 residues, 6733.33 Da.
GuHCl, non-polymer, 59.070 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: n/a
Entity Sequences (FASTA):
Fyn_SH3_domain: VTLFVALYDYEARTEDDLSF
HKGEKFQILNSSEGDWWEAR
SLTTGETGYIPSNYVAPVD
| Data type | Count |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fyn SH3 domain | 1 |
| 2 | Guanidium chloride | 2 |
Entities:
Entity 1, Fyn SH3 domain 59 residues - 6733.33 Da.
| 1 | VAL | THR | LEU | PHE | VAL | ALA | LEU | TYR | ASP | TYR | ||||
| 2 | GLU | ALA | ARG | THR | GLU | ASP | ASP | LEU | SER | PHE | ||||
| 3 | HIS | LYS | GLY | GLU | LYS | PHE | GLN | ILE | LEU | ASN | ||||
| 4 | SER | SER | GLU | GLY | ASP | TRP | TRP | GLU | ALA | ARG | ||||
| 5 | SER | LEU | THR | THR | GLY | GLU | THR | GLY | TYR | ILE | ||||
| 6 | PRO | SER | ASN | TYR | VAL | ALA | PRO | VAL | ASP |
Entity 2, Guanidium chloride - . - 59.070 Da.
| 1 | . |
Samples:
sample_1: Fyn SH3 domain, [U-100% 15N], 0.25 mM; sodium phosphate 50 mM; NaCl 100 mM; EDTA 0.2 mM; DSS 4 mM; GuHCl0 – 0.85 M
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking
Molmol, Koradi, Billeter and Wuthrich - processing
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAE33766 BAE42585 BAG70107 BAG70240 BAI46902 |
| EMBL | CAA37025 CAC44028 |
| GB | AAA36615 AAA37644 AAA82942 AAB09568 AAB33113 |
| REF | NP_001071440 NP_001073675 NP_001116364 NP_001116365 NP_002028 |
| SP | A0JNB0 A1Y2K1 P06241 P39688 Q05876 |
| TPG | DAA26259 |
| AlphaFold | P06241 A0JNB0 A1Y2K1 P39688 Q05876 |