BMRB Entry 16446
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16446
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Title: Chemical shift assignments for Ncs1p PubMed: 19851889
Deposition date: 2009-08-10 Original release date: 2009-11-19
Authors: Ames, James
Citation: Lim, Sunghyuk; Ames, James. "(1)H, (15)N, and (13)C chemical shift assignments of neuronal calcium sensor-1 homolog from fission yeast." Biomol NMR Assign 3, 269-271 (2009).
Assembly members:
Ncs1p, polymer, 190 residues, Formula weight is not available
Natural source: Common Name: Fission Yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
Ncs1p: MGKSQSKLSQDQLQDLVRST
RFDKKELQQWYKGFFKDCPS
GHLNKSEFQKIYKQFFPFGD
PSAFAEYVFNVFDADKNGYI
DFKEFICALSVTSRGELNDK
LIWAFQLYDLDNNGLISYDE
MLRIVDAIYKMVGSMVKLPE
DEDTPEKRVNKIFNMMDKNK
DGQLTLEEFCEGSKRDPTIV
SALSLYDGLV
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 600 |
15N chemical shifts | 184 |
1H chemical shifts | 1034 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 190 residues - Formula weight is not available
1 | MET | GLY | LYS | SER | GLN | SER | LYS | LEU | SER | GLN | |
2 | ASP | GLN | LEU | GLN | ASP | LEU | VAL | ARG | SER | THR | |
3 | ARG | PHE | ASP | LYS | LYS | GLU | LEU | GLN | GLN | TRP | |
4 | TYR | LYS | GLY | PHE | PHE | LYS | ASP | CYS | PRO | SER | |
5 | GLY | HIS | LEU | ASN | LYS | SER | GLU | PHE | GLN | LYS | |
6 | ILE | TYR | LYS | GLN | PHE | PHE | PRO | PHE | GLY | ASP | |
7 | PRO | SER | ALA | PHE | ALA | GLU | TYR | VAL | PHE | ASN | |
8 | VAL | PHE | ASP | ALA | ASP | LYS | ASN | GLY | TYR | ILE | |
9 | ASP | PHE | LYS | GLU | PHE | ILE | CYS | ALA | LEU | SER | |
10 | VAL | THR | SER | ARG | GLY | GLU | LEU | ASN | ASP | LYS | |
11 | LEU | ILE | TRP | ALA | PHE | GLN | LEU | TYR | ASP | LEU | |
12 | ASP | ASN | ASN | GLY | LEU | ILE | SER | TYR | ASP | GLU | |
13 | MET | LEU | ARG | ILE | VAL | ASP | ALA | ILE | TYR | LYS | |
14 | MET | VAL | GLY | SER | MET | VAL | LYS | LEU | PRO | GLU | |
15 | ASP | GLU | ASP | THR | PRO | GLU | LYS | ARG | VAL | ASN | |
16 | LYS | ILE | PHE | ASN | MET | MET | ASP | LYS | ASN | LYS | |
17 | ASP | GLY | GLN | LEU | THR | LEU | GLU | GLU | PHE | CYS | |
18 | GLU | GLY | SER | LYS | ARG | ASP | PRO | THR | ILE | VAL | |
19 | SER | ALA | LEU | SER | LEU | TYR | ASP | GLY | LEU | VAL |
Samples:
sample_1: Ncs1p, [U-95% 13C; U-95% 15N], 1.0 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.02 M; pH: 7.0; pressure: 1 atm; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts