BMRB Entry 16450

Name: Backbone and side chain 1H, 13C, 15N chemical shift assignments for YSK2
Published: 2009-11-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16450

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone and side chain 1H, 13C, 15N chemical shift assignments for YSK2 PubMed: 19842064

Deposition date: 2009-08-17 Original release date: 2009-11-16

Authors: Findlater, Emma; Steffen, Graether

Citation: Findlater, Emma; Graether, Steffen. "NMR assignments of the intrinsically disordered K(2) and YSK (2) dehydrins" Biomol. NMR Assignments 3, 273-275 (2009).

Assembly members:
YSK2, polymer, 130 residues, Formula weight is not available

Natural source: Common Name: eudicots Taxonomy ID: 96939 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Vitis riparia

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):
YSK2: MAYQQDPCANPTRQTGKTGG QTDQYGNPVHQTEALGAYGA GTGTGMHGGEHQQQPHQQPG VLNRSGSSSSEDDGQGGRRK KGMKEKIKERIPGMGRKDEQ KQTSATSTPGQGQQQKGMME KIKEKLPGAH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	398
15N chemical shifts	109
1H chemical shifts	615

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	YSK2 monomer	1

Entities:

Entity 1, YSK2 monomer 130 residues - Formula weight is not available

1	MET	ALA	TYR	GLN	GLN	ASP	PRO	CYS	ALA	ASN
2	PRO	THR	ARG	GLN	THR	GLY	LYS	THR	GLY	GLY
3	GLN	THR	ASP	GLN	TYR	GLY	ASN	PRO	VAL	HIS
4	GLN	THR	GLU	ALA	LEU	GLY	ALA	TYR	GLY	ALA
5	GLY	THR	GLY	THR	GLY	MET	HIS	GLY	GLY	GLU
6	HIS	GLN	GLN	GLN	PRO	HIS	GLN	GLN	PRO	GLY
7	VAL	LEU	ASN	ARG	SER	GLY	SER	SER	SER	SER
8	GLU	ASP	ASP	GLY	GLN	GLY	GLY	ARG	ARG	LYS
9	LYS	GLY	MET	LYS	GLU	LYS	ILE	LYS	GLU	ARG
10	ILE	PRO	GLY	MET	GLY	ARG	LYS	ASP	GLU	GLN
11	LYS	GLN	THR	SER	ALA	THR	SER	THR	PRO	GLY
12	GLN	GLY	GLN	GLN	GLN	LYS	GLY	MET	MET	GLU
13	LYS	ILE	LYS	GLU	LYS	LEU	PRO	GLY	ALA	HIS

Samples:

sample_1: YSK2, [U-100% 13C; U-100% 15N], 0.6 mM; H2O 95%; D2O 5%; NaCl 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

DBJ	BAM13716 BAM13717
GB	AAF37268 AAW58105 AEP60143 AFD62287 AFG16844

Biological Magnetic Resonance Data Bank