BMRB Entry 16452
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16452
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Title: NMR structure of the C-terminal domain of pUL89 PubMed: 16846228
Deposition date: 2009-08-18 Original release date: 2012-08-03
Authors: Couvreux, Anthony; Hantz, Sebastien; Marquant, Rodrigue; Champier, Gael; Alain, Sophie; Morellet, Nelly; Bouaziz, Serge
Citation: Thoma, Corina; Borst, Eva; Messerle, Martin; Rieger, Manuela; Hwang, Jae-Seon; Bogner, Elke. "Identification of the Interaction Domain of the Small Terminase Subunit pUL89 with the Large Subunit pUL56 of Human Cytomegalovirus" Biochemistry 45, 8855-8863 (2006).
Assembly members:
pUL89-Cter, polymer, 68 residues, 7916.990 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
pUL89-Cter: DQNHIEQPFYLMGRDKALAV
EQFISRFNSGYIKASQELVS
YTIKLSHDPIEYLLEQIQNL
HRVTLAEG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 461 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pUL89-Cter | 1 |
Entities:
Entity 1, pUL89-Cter 68 residues - 7916.990 Da.
| 1 | ASP | GLN | ASN | HIS | ILE | GLU | GLN | PRO | PHE | TYR | ||||
| 2 | LEU | MET | GLY | ARG | ASP | LYS | ALA | LEU | ALA | VAL | ||||
| 3 | GLU | GLN | PHE | ILE | SER | ARG | PHE | ASN | SER | GLY | ||||
| 4 | TYR | ILE | LYS | ALA | SER | GLN | GLU | LEU | VAL | SER | ||||
| 5 | TYR | THR | ILE | LYS | LEU | SER | HIS | ASP | PRO | ILE | ||||
| 6 | GLU | TYR | LEU | LEU | GLU | GLN | ILE | GLN | ASN | LEU | ||||
| 7 | HIS | ARG | VAL | THR | LEU | ALA | GLU | GLY |
Samples:
sample_1: pUL89-Cter 0.8 mM; H2O 50%; acetonitril 50%
sample_conditions_1: pH: 3.2; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH v2.19, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker DMX 600 MHz
- Bruker DMX 600 MHz