BMRB Entry 16460
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16460
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Title: The B12-Binding Subunit of Glutamate Mutase from Clostridium tetanomorphum Traps the Nucleotide Moiety of Coenzyme B12 PubMed: 11397096
Deposition date: 2009-08-19 Original release date: 2010-03-08
Authors: Tollinger, Martin; Eichmuller, Chritian; Konrat, Robert; Huhta, Maria; Marsh, E.; Krautler, Bernhard
Citation: Tollinger, Martin; Eichmuller, Chritian; Konrat, Robert; Huhta, Maria; Marsh, E.; Krautler, Bernhard. "The B(12)-binding subunit of glutamate mutase from Clostridium tetanomorphum traps the nucleotide moiety of coenzyme B(12).Moiety of Coenzyme B12" J. Mol. Biol. 309, 777-791 (2001).
Assembly members:
MutS, polymer, 136 residues, Formula weight is not available
B12-nucleotide, non-polymer, Formula weight is not available
Natural source: Common Name: Clostridium tetanomorphum Taxonomy ID: 1553 Superkingdom: Bacteria Kingdom: not available Genus/species: Clostridium tetanomorphum
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
MutS: MEKKTIVLGVIGSDCHAVGN
KILDHSFTNAGFNVVNIGVL
SSQEDFINAAIETKADLICV
SSLYGQGEIDCKGLREKCDE
AGLKGIKLFVGGNIVVGKQN
WPDVEQRFKAMGFDRVYPPG
TSPETTIADMKEVLGV
- binding_constants
| Data type | Count |
| binding constants | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MutS | 1 |
| 2 | B12-nucleotide | 2 |
Entities:
Entity 1, MutS 136 residues - Formula weight is not available
| 1 | MET | GLU | LYS | LYS | THR | ILE | VAL | LEU | GLY | VAL | ||||
| 2 | ILE | GLY | SER | ASP | CYS | HIS | ALA | VAL | GLY | ASN | ||||
| 3 | LYS | ILE | LEU | ASP | HIS | SER | PHE | THR | ASN | ALA | ||||
| 4 | GLY | PHE | ASN | VAL | VAL | ASN | ILE | GLY | VAL | LEU | ||||
| 5 | SER | SER | GLN | GLU | ASP | PHE | ILE | ASN | ALA | ALA | ||||
| 6 | ILE | GLU | THR | LYS | ALA | ASP | LEU | ILE | CYS | VAL | ||||
| 7 | SER | SER | LEU | TYR | GLY | GLN | GLY | GLU | ILE | ASP | ||||
| 8 | CYS | LYS | GLY | LEU | ARG | GLU | LYS | CYS | ASP | GLU | ||||
| 9 | ALA | GLY | LEU | LYS | GLY | ILE | LYS | LEU | PHE | VAL | ||||
| 10 | GLY | GLY | ASN | ILE | VAL | VAL | GLY | LYS | GLN | ASN | ||||
| 11 | TRP | PRO | ASP | VAL | GLU | GLN | ARG | PHE | LYS | ALA | ||||
| 12 | MET | GLY | PHE | ASP | ARG | VAL | TYR | PRO | PRO | GLY | ||||
| 13 | THR | SER | PRO | GLU | THR | THR | ILE | ALA | ASP | MET | ||||
| 14 | LYS | GLU | VAL | LEU | GLY | VAL |
Entity 2, B12-nucleotide - C16 H26 N3 O7 P - Formula weight is not available
Samples:
sample_1: phosphate buffer 11 mM; DTT 5 mM; MutS, [U-15N], 0.8 mM; B12-nucleotide0.4 – 25.6 mM; D2O 10%; H2O 90%
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 299 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
Molmol, Koradi, Billeter and Wuthrich - visualization
X-PLOR v3.1, Brunger - structure solution
NMR spectrometers:
- Varian UnityPlus 500 MHz