BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16466

Title: Solution structure and functional characterization of human plasminogen kringle 5   PubMed: 19821587

Deposition date: 2009-08-21 Original release date: 2009-12-11

Authors: Battistel, Marcos; Grishaev, Alexander; An, Seong Soo; Castellino, Francis; Llinas, Miguel

Citation: Battistel, Marcos; Grishaev, Alexander; An, Seong Soo A; Castellino, Francis; Llinas, Miguel. "Solution structure and functional characterization of human plasminogen kringle 5."  Biochemistry 48, 10208-10219 (2009).

Assembly members:
rK5, polymer, 86 residues, 9569.646 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: not applicable

Entity Sequences (FASTA):
rK5: SNADCMFGNGKGYRGKRVTT VTGTPCQDWAAQEPHRHSIF TPETNPRAGLEKNYCRNPDG DVGGPWCYTTNPRKLYDYCD VPQCAA

Data sets:
Data typeCount
15N chemical shifts95
1H chemical shifts469

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rK51

Entities:

Entity 1, rK5 86 residues - 9569.646 Da.

1   SERASNALAASPCYSMETPHEGLYASNGLY
2   LYSGLYTYRARGGLYLYSARGVALTHRTHR
3   VALTHRGLYTHRPROCYSGLNASPTRPALA
4   ALAGLNGLUPROHISARGHISSERILEPHE
5   THRPROGLUTHRASNPROARGALAGLYLEU
6   GLULYSASNTYRCYSARGASNPROASPGLY
7   ASPVALGLYGLYPROTRPCYSTYRTHRTHR
8   ASNPROARGLYSLEUTYRASPTYRCYSASP
9   VALPROGLNCYSALAALA

Samples:

sample_1: D2O 10%; H2O 90%; sodium phosphate 50 mM; AMCHA 11 mM; rK5 2.2 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 5.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - chemical shift assignment, refinement, structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
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