BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16502

Title: Backbone and side chain 1H chemical shift assignments for acidic fibrobast growth factor bound to 1,3,6-naphthalenetrisulfonate   PubMed: 9719643

Deposition date: 2009-09-18 Original release date: 2009-10-20

Authors: Lozano, Rosa; Jimenez, M. Angeles; Santoro, Jorge; Rico, Manuel; Gimenez-Gallego, Guillermo

Citation: Pineda-Lucena, Antonio; Jimenez, M. Angeles; Nieto, Jose; Santoro, Jorge; Rico, Manuel; Gimenez-Gallego, Guillermo. "Solution structure of acidic fibroblast growth factor bound to 1,3, 6-naphthalenetrisulfonate: a minimal model for the anti-tumoral action of suramins and suradistas."  J. Mol. Biol. 281, 899-915 (1998).

Assembly members:
aFGF, polymer, 132 residues, Formula weight is not available
NTS, non-polymer, 365.336 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMG47

Entity Sequences (FASTA):
aFGF: KKPKLLYCSNGGHFLRILPD GTVDGTRDRSDQHIQLQLSA ESVGEVYIKSTETGQYLAMD TDGLLYGSQTPNEECLFLER LEENHYNTYISKKHAEKNWF VGLKKNGSCKRGPRTHYGQK AILFLPLPVSSD

Data sets:
Data typeCount
1H chemical shifts790

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1acidic fibroblast growth factor1
21,3,6-naphthalenetrisulfonate2

Entities:

Entity 1, acidic fibroblast growth factor 132 residues - Formula weight is not available

Protein construct lacks the 22 N-terminal residues.

1   LYSLYSPROLYSLEULEUTYRCYSSERASN
2   GLYGLYHISPHELEUARGILELEUPROASP
3   GLYTHRVALASPGLYTHRARGASPARGSER
4   ASPGLNHISILEGLNLEUGLNLEUSERALA
5   GLUSERVALGLYGLUVALTYRILELYSSER
6   THRGLUTHRGLYGLNTYRLEUALAMETASP
7   THRASPGLYLEULEUTYRGLYSERGLNTHR
8   PROASNGLUGLUCYSLEUPHELEUGLUARG
9   LEUGLUGLUASNHISTYRASNTHRTYRILE
10   SERLYSLYSHISALAGLULYSASNTRPPHE
11   VALGLYLEULYSLYSASNGLYSERCYSLYS
12   ARGGLYPROARGTHRHISTYRGLYGLNLYS
13   ALAILELEUPHELEUPROLEUPROVALSER
14   SERASP

Entity 2, 1,3,6-naphthalenetrisulfonate - C10 H5 O9 S3 - 365.336 Da.

1   NTS

Samples:

sample_1: aFGF1 – 2 mM; sodium phosphate 10 mM; TSP0.1 – 0.2 mM; beta-mercaptoethanol 1 mM; sodium chloride 150 mM; NTS 2 mM; H2O 90%; D2O 10%

sample_2: aFGF1 – 2 mM; sodium phosphate 10 mM; TSP0.1 – 0.2 mM; beta-mercaptoethanol 1 mM; sodium chloride 150 mM; NTS 2 mM; D2O 100%

sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1.0 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

UXNMR, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AMX 600 MHz