BMRB Entry 16541

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16541

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Title:
Assignment of the Orphan Nuclear Receptor Nurr1 by NMR
Deposition date:
2009-10-06
Original release date:
2010-10-06
Authors:
Michiels, Paul; Atkins, Karen; Ludwig, Christian; Whittaker, Sara; vanDongen, Maria; Gunther, Ulrich
Citation:

Citation: Michiels, Paul; Atkins, Karen; Ludwig, Christian; Whittaker, Sara; van Dongen, Maria; Gunther, Ulrich. "Assignment of the orphan nuclear receptor Nurr1 by NMR."  Biomol. NMR Assignments 4, 101-105 (2010).
PubMed: 20300892

Assembly members:

Assembly members:
Nurr1, polymer, 251 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nurr1: GPLGSQEPSPPSPPVSLISA LVRAHVDSNPAMTSLDYSRF QANPDYQMSGDDTQHIQQFY DLLTGSMEIIRGWAEKIPGF ADLPKADQDLLFESAFLELF VLRLAYRSNPVEGKLIFCNG VVLHRLQCVRGFGEWIDSIV EFSSNLQNMNIDISAFSCIA ALAMVTERHGLKEPKRVEEL QNKIVNCLKDHVTFNNGGLN RPNYLSKLLGKLPELRTLCT QGLQRIFYLKLEDLVPPPAI IDKLFLDTLPF

Data sets:
Data typeCount
13C chemical shifts566
15N chemical shifts196
1H chemical shifts195

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit_11

Entities:

Entity 1, subunit_1 251 residues - Formula weight is not available

1   GLYPROLEUGLYSERGLNGLUPROSERPRO
2   PROSERPROPROVALSERLEUILESERALA
3   LEUVALARGALAHISVALASPSERASNPRO
4   ALAMETTHRSERLEUASPTYRSERARGPHE
5   GLNALAASNPROASPTYRGLNMETSERGLY
6   ASPASPTHRGLNHISILEGLNGLNPHETYR
7   ASPLEULEUTHRGLYSERMETGLUILEILE
8   ARGGLYTRPALAGLULYSILEPROGLYPHE
9   ALAASPLEUPROLYSALAASPGLNASPLEU
10   LEUPHEGLUSERALAPHELEUGLULEUPHE
11   VALLEUARGLEUALATYRARGSERASNPRO
12   VALGLUGLYLYSLEUILEPHECYSASNGLY
13   VALVALLEUHISARGLEUGLNCYSVALARG
14   GLYPHEGLYGLUTRPILEASPSERILEVAL
15   GLUPHESERSERASNLEUGLNASNMETASN
16   ILEASPILESERALAPHESERCYSILEALA
17   ALALEUALAMETVALTHRGLUARGHISGLY
18   LEULYSGLUPROLYSARGVALGLUGLULEU
19   GLNASNLYSILEVALASNCYSLEULYSASP
20   HISVALTHRPHEASNASNGLYGLYLEUASN
21   ARGPROASNTYRLEUSERLYSLEULEUGLY
22   LYSLEUPROGLULEUARGTHRLEUCYSTHR
23   GLNGLYLEUGLNARGILEPHETYRLEULYS
24   LEUGLUASPLEUVALPROPROPROALAILE
25   ILEASPLYSLEUPHELEUASPTHRLEUPRO
26   PHE

Samples:

sample_1: Nurr1, [U-100% 13C; U-100% 15N; U-80% 2H], 500 uM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.1; pressure: 1 atm; temperature: 326.7 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

NMRPipe v2009, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ANALYSIS v1.0 R15, Vranken, Boucher, Stevens, Fogh, Pajon, Llinas, Ulrich, Markley, Ionides, Laue - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA75666 BAA77328 BAD97345 BAE34579 BAF84145
EMBL CAA53518 CAH92832
GB AAA42058 AAB25138 AAB33999 AAC52143 AAC53153
REF NP_001069676 NP_001132981 NP_001177205 NP_001253839 NP_006177
SP P43354 Q06219 Q07917 Q08E53 Q5R5Y4
TPG DAA32695
AlphaFold P43354 Q06219 Q07917 Q08E53 Q5R5Y4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks