BMRB Entry 16614

Name: 1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity
Published: 2010-05-05

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16614

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity

Deposition date:

2009-11-22

Original release date:

2010-05-05

Authors:

Yao, Shenggen; Murphy, James; Low, Andrew; Norton, Raymond

Citation:

Citation: Yao, Shenggen; Murphy, James; Low, Andrew; Norton, Raymond. "1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity." Biomol. NMR Assignments 4, 73-77 (2010).
PubMed: 20174897

Assembly members:

Assembly members:
mIL3, polymer, 127 residues, 14286 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM60

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mIL3: GAMDTHRLTRTLNCSSIVKE IIGKLPEPELKTDDEGPSLR NKSFRRVNLSKFVESQGEVD PEDRYVIKSNLQKLNACLPT SANDSALPGVFIRDLDDFRK KLRFYMVHLNDLETVLTSRP PQPASGS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	460
15N chemical shifts	125
1H chemical shifts	793

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mIL3(33-156)	1

Entities:

Entity 1, mIL3(33-156) 127 residues - 14286 Da.

The first 3 residues are cloning artifacts

1

GLY

ALA

MET

ASP

THR

HIS

ARG

LEU

THR

ARG

2

THR

LEU

ASN

CYS

SER

ILE

VAL

LYS

GLU

3

ILE

GLY

LYS

LEU

PRO

GLU

PRO

GLU

LEU

4

LYS

THR

ASP

GLU

GLY

PRO

SER

LEU

ARG

5

ASN

LYS

SER

PHE

ARG

VAL

ASN

LEU

SER

6

LYS

PHE

VAL

GLU

SER

GLN

GLY

GLU

VAL

ASP

7

PRO

GLU

ASP

ARG

TYR

VAL

ILE

LYS

SER

ASN

8

LEU

GLN

LYS

LEU

ASN

ALA

CYS

LEU

PRO

THR

9

SER

ALA

ASN

ASP

SER

ALA

LEU

PRO

GLY

VAL

10

PHE

ILE

ARG

ASP

LEU

ASP

PHE

ARG

LYS

11

LYS

LEU

ARG

PHE

TYR

MET

VAL

HIS

LEU

ASN

12

ASP

LEU

GLU

THR

VAL

LEU

THR

SER

ARG

PRO

13

PRO

GLN

PRO

ALA

SER

GLY

SER

Samples:

sample_1: mIL3, [U-100% 15N], 0.28 mM; potassium phosphate 20 mM; sodium azide 0.2%; H2O 95%; D2O 5%

sample_2: mIL3, [U-100% 13C; U-100% 15N], 0.28 mM; potassium phosphate 20 mM; sodium azide 0.2%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.7; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - processing

XEASY v1.3, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 800 MHz

PDB	2L3O
DBJ	BAE32129
EMBL	CAA26514
GB	AAA39291 AAA39293 AAA39308 AAA39507 AAB24323
PRF	1002253A
REF	NP_034686
SP	P01586
AlphaFold	P01586