BMRB Entry 16666
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16666
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Sequence-specific 1H, 15N and 13C resonance assignments of Sugarcane defensin 5 (Sd5). PubMed: 21220113
Deposition date: 2010-01-05 Original release date: 2011-03-31
Authors: de Paula, Viviane; Almeida, Fabio; Valente, Ana Paula
Citation: De Paula, Viviane; Razzera, Guilherme; Barreto-Bergter, Eliana; Almeida, Fabio; Valente, Ana Paula. "Portrayal of complex dynamic properties of sugarcane defensin 5 by NMR: multiple motions associated with membrane interaction" Structure 19, 26-36 (2011).
Assembly members:
Sd5-_Sugarcane_defensin_5, polymer, 71 residues, Formula weight is not available
Natural source: Common Name: Sugarcane Taxonomy ID: 4547 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Saccharum officinarum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a
Entity Sequences (FASTA):
Sd5-_Sugarcane_defensin_5: HTPTPTPICKSRSHEYKGRC
IQDMDCNAACVKESESYTGG
FCNGRPPFKQCFCTKPCKRE
RAAATLRWPGL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 270 |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 449 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sd5 | 1 |
Entities:
Entity 1, Sd5 71 residues - Formula weight is not available
| 1 | HIS | THR | PRO | THR | PRO | THR | PRO | ILE | CYS | LYS | ||||
| 2 | SER | ARG | SER | HIS | GLU | TYR | LYS | GLY | ARG | CYS | ||||
| 3 | ILE | GLN | ASP | MET | ASP | CYS | ASN | ALA | ALA | CYS | ||||
| 4 | VAL | LYS | GLU | SER | GLU | SER | TYR | THR | GLY | GLY | ||||
| 5 | PHE | CYS | ASN | GLY | ARG | PRO | PRO | PHE | LYS | GLN | ||||
| 6 | CYS | PHE | CYS | THR | LYS | PRO | CYS | LYS | ARG | GLU | ||||
| 7 | ARG | ALA | ALA | ALA | THR | LEU | ARG | TRP | PRO | GLY | ||||
| 8 | LEU |
Samples:
sample_1: Sd5- Sugarcane defensin 5, [U-99% 15N], 1 mM; sodium phophate buffer 5 mM; H2O 90%; D2O 10%
sample_2: Sd5- Sugarcane defensin 5, [U-99% 13C; U-99% 15N], 0.7 mM; sodium phophate buffer 5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.005 M; pH: 4.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts