BMRB Entry 16687

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16687

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Title:
1H, 13C and 15N assignments of the dimeric regulatory subunit (ilvN) of E.coli AHAS I
Deposition date:
2010-01-21
Original release date:
2010-05-05
Authors:
Karanth, Megha; Sarma, Siddhartha
Citation:

Citation: Karanth, N. Megha; Sarma, Siddhartha. "1H, 13C, 15N assignments of the dimeric regulatory subunit (ilvN) of the E. coli AHAS I."  Biomol. NMR Assignments 4, 131-133 (2010).
PubMed: 20383786

Assembly members:

Assembly members:
ilvN, polymer, 98 residues, Formula weight is not available
VAL, polymer, . residues, 117.146 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ilvN: GSMQNTTHDNVILELTVRNH PGVMTHVCGLFARRAFNVEG ILCLPIQDSDKSHIWLLVND DQRLEQMISQIDKLEDVVKV QRNQSDPTMFNKIAVFFQ

Data sets:
Data typeCount
13C chemical shifts387
15N chemical shifts98
1H chemical shifts647

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ilvN polypeptide 11
2L-Valine 12
3ilvN polypeptide 21
4L-Valine 22

Entities:

Entity 1, ilvN polypeptide 1 98 residues - Formula weight is not available

Above sequence represents the true amino acid sequene as encoded by the ilvN gene without any cloning artifacts

1   GLYSERMETGLNASNTHRTHRHISASPASN
2   VALILELEUGLULEUTHRVALARGASNHIS
3   PROGLYVALMETTHRHISVALCYSGLYLEU
4   PHEALAARGARGALAPHEASNVALGLUGLY
5   ILELEUCYSLEUPROILEGLNASPSERASP
6   LYSSERHISILETRPLEULEUVALASNASP
7   ASPGLNARGLEUGLUGLNMETILESERGLN
8   ILEASPLYSLEUGLUASPVALVALLYSVAL
9   GLNARGASNGLNSERASPPROTHRMETPHE
10   ASNLYSILEALAVALPHEPHEGLN

Entity 2, L-Valine 1 - 117.146 Da.

1   VAL

Samples:

sample_1: ilvN, [U-99% 15N], 0.4 mM; potassium phosphate 20 mM; sodium chloride 20 mM; EDTA 1 mM; sodium azide 0.01%; L-Valine 5 mM; H2O 90%; D2O 10%

sample_2: ilvN, [U-99% 13C; U-99% 15N], 0.4 mM; potassium phosphate 20 mM; sodium chloride 20 mM; EDTA 1 mM; sodium azide 0.01%; L-Valine 5 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D trHNCACBsample_2isotropicsample_conditions_1
3D trCBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_2isotropicsample_conditions_1
2D tr 1H-13C HSQC (AROMATIC)sample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ANSIG, Kraulis - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 18591
PDB
DBJ BAB38034 BAE77623 BAG79478 BAI28069 BAI38251
EMBL CAA26388 CAP78134 CAQ34013 CAQ91401 CAR00641
GB AAA62022 AAC76693 AAG58873 AAN45231 AAN83029
REF NP_312638 NP_418126 NP_709524 WP_001181704 WP_001181705
SP P0ADF8 P0ADF9 P0ADG0
AlphaFold P0ADF8 P0ADF9 P0ADG0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks