BMRB Entry 16721
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16721
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Title: Solution structure of BRD1 PHD1 finger
Deposition date: 2010-02-12 Original release date: 2012-08-03
Authors: Zhang, Jiahai; Wu, Jihui; Shi, Yunyu
Citation: Qin, Su; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu. "Solution structure of BRD1 PHD1 finger" .
Assembly members:
BRD1 PHD1 finger, polymer, 71 residues, 6966.939 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p28
Entity Sequences (FASTA):
BRD1 PHD1 finger: MGHHHHHHMQSLIDEDAVCS
ICMDGESQNSNVILFCDMCN
LAVHQECYGVPYIPEGQWLC
RHCLQSRARPA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 223 |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 385 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BRD1 PHD1 finger | 1 |
| 2 | ZINC ION_1 | 2 |
| 3 | ZINC ION_2 | 2 |
Entities:
Entity 1, BRD1 PHD1 finger 71 residues - 6966.939 Da.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | MET | GLN | ||||
| 2 | SER | LEU | ILE | ASP | GLU | ASP | ALA | VAL | CYS | SER | ||||
| 3 | ILE | CYS | MET | ASP | GLY | GLU | SER | GLN | ASN | SER | ||||
| 4 | ASN | VAL | ILE | LEU | PHE | CYS | ASP | MET | CYS | ASN | ||||
| 5 | LEU | ALA | VAL | HIS | GLN | GLU | CYS | TYR | GLY | VAL | ||||
| 6 | PRO | TYR | ILE | PRO | GLU | GLY | GLN | TRP | LEU | CYS | ||||
| 7 | ARG | HIS | CYS | LEU | GLN | SER | ARG | ALA | ARG | PRO | ||||
| 8 | ALA |
Entity 2, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: ZINC ION 2 mM; entity_1, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 150 mM; Bis-Tris 20 mM; H2O 90%; D2O 10%
sample_2: ZINC ION 2 mM; entity_1, [U-100% 13C; U-100% 15N], 1 mM; Bis-Tris 20 mM; sodium chloride 150 mM; D2O 100%
sample_conditions_1: ionic strength: 0.15 M; pH: 6.7; pressure: 1 atm; temperature: 293 K
sample_conditions_2: ionic strength: 0.15 M; pD: 6.7; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
NMRPipe v2.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3, Goddard - chemical shift assignment, data analysis
CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution
TALOS, Cornilescu, Delaglio and Bax - processing
Molmol v2K.2, Koradi, Billeter and Wuthrich - data analysis
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
| BMRB | 17244 |
| PDB | |
| REF | XP_009232775 XP_009232776 XP_009232777 XP_009232778 XP_009232779 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts