BMRB Entry 16776

Name: C-terminal domain of mesencephalic astrocyte-derived neurotrophic factor (MANF)
Published: 2022-02-14

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PDB ID: 2kve
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16776
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: C-terminal domain of mesencephalic astrocyte-derived neurotrophic factor (MANF) PubMed: 21047780

Deposition date: 2010-03-12 Original release date: 2022-02-14

Authors: Hellman, Maarit; Saarma, Mart; Permi, Perttu

Citation: Hellman, Maarit; Arumae, Urmas; Yu, Li-ying; Lindholm, Paivi; Peranen, Johan; Saarma, Mart; Permi, Perttu. "Mesencephalic astrocyte-derived neurotrophic factor (MANF) has a unique mechanism to rescue apoptotic neurons" J. Biol. Chem. 286, 2675-2680 (2011).

Assembly members:
C-MANF, polymer, 65 residues, 7476.837 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):
C-MANF: MGKYDKQIDLSTVDLKKLRV KELKKILDDWGETCKGCAEK SDYIRKINELMPKYAPKAAS ARTDL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	227
15N chemical shifts	61
1H chemical shifts	461

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-MANF	1

Entities:

Entity 1, C-MANF 65 residues - 7476.837 Da.

First two residues are cloning artefacts

1

MET

GLY

LYS

TYR

ASP

LYS

GLN

ILE

ASP

LEU

2

SER

THR

VAL

ASP

LEU

LYS

LEU

ARG

VAL

3

LYS

GLU

LEU

LYS

ILE

LEU

ASP

TRP

4

GLY

GLU

THR

CYS

LYS

GLY

CYS

ALA

GLU

LYS

5

SER

ASP

TYR

ILE

ARG

LYS

ILE

ASN

GLU

LEU

6

MET

PRO

LYS

TYR

ALA

PRO

LYS

ALA

SER

7

ALA

ARG

THR

ASP

LEU

Samples:

sample_1: C-MANF, [U-99% 13C; U-99% 15N], 1 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C-CT-HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
2D (HB)CB(CGCD)HD	sample_1	isotropic	sample_conditions_1
2D (HB)CB(CGCDCE)HE	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.0.26, P.GUNTERT ET AL. - automated NOE assignment

AMBER v8.0, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement, structure solution

NMR spectrometers:

Varian Unity Inova 600 MHz
Varian Unity Inova 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts