BMRB Entry 16803
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16803
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Title: 1H, 13C and 15N backbone and side chain resonance assignment of the human RANTES-E66S mutant in a dimeric form
Deposition date: 2010-03-30 Original release date: 2010-04-28
Authors: Bieri, Andrej; Grzesiek, Stephan
Citation: Bieri, Andrej; Grzesiek, Stephan. "1H, 13C and 15N backbone and side chain resonance assignment of the human RANTES-E66S mutant in a dimeric form" .
Assembly members:
RANTES-E66S, polymer, 68 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pEGV2
Entity Sequences (FASTA):
RANTES-E66S: SPYSSDTTPCCFAYIARPLP
RAHIKEYFYTSGKCSNPAVV
FVTRKNRQVCANPEKKWVRE
YINSLSMS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 310 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 473 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | polymer | 1 |
Entities:
Entity 1, polymer 68 residues - Formula weight is not available
| 1 | SER | PRO | TYR | SER | SER | ASP | THR | THR | PRO | CYS | ||||
| 2 | CYS | PHE | ALA | TYR | ILE | ALA | ARG | PRO | LEU | PRO | ||||
| 3 | ARG | ALA | HIS | ILE | LYS | GLU | TYR | PHE | TYR | THR | ||||
| 4 | SER | GLY | LYS | CYS | SER | ASN | PRO | ALA | VAL | VAL | ||||
| 5 | PHE | VAL | THR | ARG | LYS | ASN | ARG | GLN | VAL | CYS | ||||
| 6 | ALA | ASN | PRO | GLU | LYS | LYS | TRP | VAL | ARG | GLU | ||||
| 7 | TYR | ILE | ASN | SER | LEU | SER | MET | SER |
Samples:
sample_1: D2O 5%; H2O 95%; potassium phosphate 50 mM; sodium azide 0.02%; Rantes-E66S, [U-100% 13C; U-100% 15N], 2 mM
sample_2: D2O 100%; potassium phosphate 50 mM; Rantes-E66S, [U-100% 15N], 0.5 mM
sample_conditions_1: pH: 3.8; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 17453 |
| PDB | |
| DBJ | BAG35145 BAG73001 |
| GB | AAA36725 AAC03541 AAC63331 AAF73070 AAH08600 |
| REF | NP_002976 XP_002827318 XP_003281422 XP_003818023 XP_008009272 |
| SP | P13501 |
| AlphaFold | P13501 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts