BMRB Entry 16937
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16937
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Title: apoWCBD4-6 PubMed: 20799727
Deposition date: 2010-05-18 Original release date: 2010-09-08
Authors: Fatemi, Negah
Citation: Fatemi, Negah; Korzhnev, Dmitry; Velyvis, Algirdas; Sarkar, Bibudhendra; Forman-Kay, Julie. "NMR characterization of copper-binding domains 4-6 of ATP7B ." Biochemistry 49, 8468-8477 (2010).
Assembly members:
apoWCBD4-6, polymer, 281 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET SUMO
Entity Sequences (FASTA):
apoWCBD4-6: GPLGSTCSTTLIAIAGMTCA
SCVHSIEGMISQLEGVQQIS
VSLAEGTATVLYNPAVISPE
ELRAAIEDMGFEASVVSESC
STNPLGNHSAGNSMVQTTDG
TPTSLQEVAPHTGRLPANHA
PDILAKSPQSTRAVAPQKCF
LQIKGMTCASCVSNIERNLQ
KEAGVLSVLVALMAGKAEIK
YDPEVIQPLEIAQFIQDLGF
EAAVMEDYAGSDGNIELTIT
GMTCASCVHNIESKLTRTNG
ITYASVALATSKALVKFDPE
IIGPRDIIKIIEEIGFHASL
A
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 667 |
| 15N chemical shifts | 227 |
| 1H chemical shifts | 227 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apoWCBD4-6 | 1 |
Entities:
Entity 1, apoWCBD4-6 281 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | THR | CYS | SER | THR | THR | ||||
| 2 | LEU | ILE | ALA | ILE | ALA | GLY | MET | THR | CYS | ALA | ||||
| 3 | SER | CYS | VAL | HIS | SER | ILE | GLU | GLY | MET | ILE | ||||
| 4 | SER | GLN | LEU | GLU | GLY | VAL | GLN | GLN | ILE | SER | ||||
| 5 | VAL | SER | LEU | ALA | GLU | GLY | THR | ALA | THR | VAL | ||||
| 6 | LEU | TYR | ASN | PRO | ALA | VAL | ILE | SER | PRO | GLU | ||||
| 7 | GLU | LEU | ARG | ALA | ALA | ILE | GLU | ASP | MET | GLY | ||||
| 8 | PHE | GLU | ALA | SER | VAL | VAL | SER | GLU | SER | CYS | ||||
| 9 | SER | THR | ASN | PRO | LEU | GLY | ASN | HIS | SER | ALA | ||||
| 10 | GLY | ASN | SER | MET | VAL | GLN | THR | THR | ASP | GLY | ||||
| 11 | THR | PRO | THR | SER | LEU | GLN | GLU | VAL | ALA | PRO | ||||
| 12 | HIS | THR | GLY | ARG | LEU | PRO | ALA | ASN | HIS | ALA | ||||
| 13 | PRO | ASP | ILE | LEU | ALA | LYS | SER | PRO | GLN | SER | ||||
| 14 | THR | ARG | ALA | VAL | ALA | PRO | GLN | LYS | CYS | PHE | ||||
| 15 | LEU | GLN | ILE | LYS | GLY | MET | THR | CYS | ALA | SER | ||||
| 16 | CYS | VAL | SER | ASN | ILE | GLU | ARG | ASN | LEU | GLN | ||||
| 17 | LYS | GLU | ALA | GLY | VAL | LEU | SER | VAL | LEU | VAL | ||||
| 18 | ALA | LEU | MET | ALA | GLY | LYS | ALA | GLU | ILE | LYS | ||||
| 19 | TYR | ASP | PRO | GLU | VAL | ILE | GLN | PRO | LEU | GLU | ||||
| 20 | ILE | ALA | GLN | PHE | ILE | GLN | ASP | LEU | GLY | PHE | ||||
| 21 | GLU | ALA | ALA | VAL | MET | GLU | ASP | TYR | ALA | GLY | ||||
| 22 | SER | ASP | GLY | ASN | ILE | GLU | LEU | THR | ILE | THR | ||||
| 23 | GLY | MET | THR | CYS | ALA | SER | CYS | VAL | HIS | ASN | ||||
| 24 | ILE | GLU | SER | LYS | LEU | THR | ARG | THR | ASN | GLY | ||||
| 25 | ILE | THR | TYR | ALA | SER | VAL | ALA | LEU | ALA | THR | ||||
| 26 | SER | LYS | ALA | LEU | VAL | LYS | PHE | ASP | PRO | GLU | ||||
| 27 | ILE | ILE | GLY | PRO | ARG | ASP | ILE | ILE | LYS | ILE | ||||
| 28 | ILE | GLU | GLU | ILE | GLY | PHE | HIS | ALA | SER | LEU | ||||
| 29 | ALA |
Samples:
sample_1: apoWCBD4-6, [U-100% 13C; U-100% 15N], 450 – 500 uM; D2O 100%
sample_conditions_1: ionic strength: 0.19 M; pH: 6; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| (H)CC(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian Unity INOVA 500 MHz
- Varian Unity INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts