BMRB Entry 16958

Name: 1H, 13C and 15N chemical shift assignments for ZCCHC9
Published: 2010-08-19

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16958

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical shift assignments for ZCCHC9

Deposition date:

2010-05-26

Original release date:

2010-08-19

Authors:

Stefl, Richard; Sanudo, Maria; Jacko, Martin; Vanacova, Stepanka

Citation:

Citation: Sanudo, Maria; Jacko, Martin; Rammelt, Christiane; Vanacova, Stepanka; Stefl, Richard. "(1)H, (13)C, and (15)N chemical shift assignments of ZCCHC9." Biomol. NMR Assignments 5, 19-21 (2011).
PubMed: 20711761

Assembly members:

Assembly members:
ZCCHC9, polymer, 164 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ZCCHC9: MQYKLILNGKTLKGETTTEA VDAATAEKVFKQYANDNGVD GEWTYDDATKTFTVTEGSNA MVCFHCRKPGHGIADCPAAL ENQDMGTGICYRCGSTEHEI TKCKAKVDPALGEFPFAKCF VCGEMGHLSRSCPDNPKGLY ADGGGCKLCGSVEHLKKDCP ESQN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	415
15N chemical shifts	152
1H chemical shifts	809

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ZCCHC9	1
2	Zn ion	2

Entities:

Entity 1, ZCCHC9 164 residues - Formula weight is not available

1

MET

GLN

TYR

LYS

LEU

ILE

LEU

ASN

GLY

LYS

2

THR

LEU

LYS

GLY

GLU

THR

GLU

ALA

3

VAL

ASP

ALA

THR

ALA

GLU

LYS

VAL

PHE

4

LYS

GLN

TYR

ALA

ASN

ASP

ASN

GLY

VAL

ASP

5

GLY

GLU

TRP

THR

TYR

ASP

ALA

THR

LYS

6

THR

PHE

THR

VAL

THR

GLU

GLY

SER

ASN

ALA

7

MET

VAL

CYS

PHE

HIS

CYS

ARG

LYS

PRO

GLY

8

HIS

GLY

ILE

ALA

ASP

CYS

PRO

ALA

LEU

9

GLU

ASN

GLN

ASP

MET

GLY

THR

GLY

ILE

CYS

10

TYR

ARG

CYS

GLY

SER

THR

GLU

HIS

GLU

ILE

11

THR

LYS

CYS

LYS

ALA

LYS

VAL

ASP

PRO

ALA

12

LEU

GLY

GLU

PHE

PRO

PHE

ALA

LYS

CYS

PHE

13

VAL

CYS

GLY

GLU

MET

GLY

HIS

LEU

SER

ARG

14

SER

CYS

PRO

ASP

ASN

PRO

LYS

GLY

LEU

TYR

15

ALA

ASP

GLY

CYS

LYS

LEU

CYS

GLY

16

SER

VAL

GLU

HIS

LEU

LYS

ASP

CYS

PRO

17

GLU

SER

GLN

ASN

Entity 2, Zn ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: ZCCHC9, [U-99% 13C; U-99% 15N], 2 mM; H2O 90%; D2O 10%; Tris buffer 20 mM; NaCl 200 mM; ZnSO4 100 %u03BCm; 2-mercaptoethanol 14 mM

sample_conditions_1: ionic strength: 0.3 M; pH: 8; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - chemical shift assignment

NMR spectrometers:

Bruker DRX 600 MHz

DBJ	BAG36844
EMBL	CAC21654 CAH92203
GB	AAH32736 AIC57311 AIC60924 EAW95870 EAW95871
REF	NP_001124507 NP_001124508 NP_001127522 NP_001182668 NP_115656
SP	Q8N567
AlphaFold	Q8N567