BMRB Entry 16962

Name: 1J and 2J coupling constants in human oxidized ERp18
Published: 2011-01-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16962

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1J and 2J coupling constants in human oxidized ERp18

Deposition date:

2010-05-27

Original release date:

2011-01-19

Authors:

Schmidt, Jurgen; Lohr, Frank

Citation:

Citation: Schmidt, Jurgen; Zhou, Shen; Rowe, Michelle; Howard, Mark; Williamson, Richard; Lohr, Frank. "One-bond and two-bond j couplings help annotate protein secondary-structure motifs: J-coupling indexing applied to human endoplasmic reticulum protein ERp18." Proteins 79, 428-443 (2011).
PubMed: 21117079

Assembly members:

Assembly members:
ERp18, polymer, 157 residues, 17774 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHIA128

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ERp18: MHHHHHHMSDGHNGLGKGFG DHIHWRTLEDGKKEAAASGL PLMVIIHKSWCGACKALKPK FAESTEISELSHNFVMVNLE DEEEPKDEDFSPDGGYIPRI LFLDPSGKVHPEIINENGNP SYKYFYVSAEQVVQGMKEAQ ERLTGDAFRKKHLEDEL

Data sets:

coupling_constants

Data type	Count
coupling constants	1268

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ERp1	1

Entities:

Entity 1, ERp1 157 residues - 17774 Da.

The actual protein sequence is preceded by an N-terminal His-tag (MHHHHHHM).

1

MET

HIS

MET

SER

ASP

2

GLY

HIS

ASN

GLY

LEU

GLY

LYS

GLY

PHE

GLY

3

ASP

HIS

ILE

HIS

TRP

ARG

THR

LEU

GLU

ASP

4

GLY

LYS

GLU

ALA

SER

GLY

LEU

5

PRO

LEU

MET

VAL

ILE

HIS

LYS

SER

TRP

6

CYS

GLY

ALA

CYS

LYS

ALA

LEU

LYS

PRO

LYS

7

PHE

ALA

GLU

SER

THR

GLU

ILE

SER

GLU

LEU

8

SER

HIS

ASN

PHE

VAL

MET

VAL

ASN

LEU

GLU

9

ASP

GLU

PRO

LYS

ASP

GLU

ASP

PHE

10

SER

PRO

ASP

GLY

TYR

ILE

PRO

ARG

ILE

11

LEU

PHE

LEU

ASP

PRO

SER

GLY

LYS

VAL

HIS

12

PRO

GLU

ILE

ASN

GLU

ASN

GLY

ASN

PRO

13

SER

TYR

LYS

TYR

PHE

TYR

VAL

SER

ALA

GLU

14

GLN

VAL

GLN

GLY

MET

LYS

GLU

ALA

GLN

15

GLU

ARG

LEU

THR

GLY

ASP

ALA

PHE

ARG

LYS

16

LYS

HIS

LEU

GLU

ASP

GLU

LEU

Name	Sample	Sample state	Sample conditions
3D Ca-coupled [15N,1H]-TROSY-HNCO	doubly_labeled	isotropic	sample_conditions_1
3D [1Ha,13Ca]-multiple-quantum HCAN quantitative J-correlation	doubly_labeled	isotropic	sample_conditions_1
3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA	doubly_labeled	isotropic	sample_conditions_1
3D Ca-coupled H(CACO)NH	doubly_labeled	isotropic	sample_conditions_1
3D Ha-coupled [15N,1H]-TROSY-H(N)COCA	doubly_labeled	isotropic	sample_conditions_1
3D Ha-coupled [15N,1H]-TROSY-HN(CO)CA	doubly_labeled	isotropic	sample_conditions_1
2D ct-[13C,1H]-IPAP-HSQC	doubly_labeled	isotropic	sample_conditions_1
2D IPAP-type HN(CO-a/b-NCa-J)-TROSY	doubly_labeled	isotropic	sample_conditions_1
2D IPAP-type HN(a/b-NCO-J)-TROSY	doubly_labeled	isotropic	sample_conditions_1
2D [15N,1H]-IPAP-HSQC	singly_labeled	isotropic	sample_conditions_1

BMRB	15964
PDB	2K8V
DBJ	BAG52132
GB	AAD20035 AAH01493 AAH08913 AAH08953 AAN34781
REF	NP_001253090 NP_056997 XP_001110583 XP_002915769 XP_003364421
SP	O95881
AlphaFold	O95881

BMRB Entry 16962

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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